Anti-TCR gamma + TCR delta antibody [TCR1] (ab25347)

Overview

  • Product nameAnti-TCR gamma + TCR delta antibody [TCR1]
    See all TCR gamma + TCR delta primary antibodies
  • Description
    Mouse monoclonal [TCR1] to TCR gamma + TCR delta
  • Specificityab25347 recognises Chicken TCR? and TCRd
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab25347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
IP
Functional Studies
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    FuncS (In ovo depletion of gamma delta T cells): Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceT cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one a and one b chain or one d and one g chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. The g/d TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most g/d T cells are CD4-/CD8-, some are CD8+. T cells expressing the g/d TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease.
    • Cellular localizationType I membrane protein
    • Alternative names
      • CD_antigen=CD3d antibody
      • CD3D antibody
      • CD3G antibody
      • T cell antigen receptor delta polypeptide antibody
      • T cell antigen receptor gamma polypeptide antibody
      • T cell rearranging gene gamma antibody
      • T cell receptor delta antibody
      • T cell receptor delta locus antibody
      • T cell receptor gamma antibody
      • T cell receptor gamma chain C region PT gamma 1/2 antibody
      • T cell receptor gamma cluster antibody
      • T cell receptor gamma locus antibody
      • T cell receptor T3 delta chain antibody
      • T cell receptor, delta (V,D,J,C) antibody
      • T cell receptors antibody
      • T cell surface glycoprotein CD3 delta chain antibody
      • T-cell receptor T3 gamma chain antibody
      • T-cell surface glycoprotein CD3 gamma chain antibody
      • T3D antibody
      • T3G antibody
      • TCRD antibody
      • TCRDV1 antibody
      • TCRG antibody
      • TRD antibody
      • TRD locus antibody
      • TRD@ antibody
      • TRG antibody
      • TRG locus antibody
      • TRGC2 antibody
      see all

    References for Anti-TCR gamma + TCR delta antibody [TCR1] (ab25347)

    This product has been referenced in:
    • Cihak J  et al. In vivo depletion of chicken T-cell subsets. Scand J Immunol 38:123-9 (1993). Read more (PubMed: 8346411) »
    • Quere P  et al. Characterization of suppressor T cells for antibody production by chicken spleen cells. I. Antigen-induced suppressor cells are CT8+, TcR1+ (gamma delta) T cells. Immunology 71:517-22 (1990). Read more (PubMed: 2149122) »

    See all 4 Publications for this product

    Product Wall

    Thank you for contacting us.

    The primary antibodies ab25345 and ab25347 you purchased are purified antibodies. We recommend to start with a concentration of 5 ug/ml for ICC and IHC. According to the results the concentration might need some op...

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    Thank you for contacting us.

    1. Yes, a substrate is needed.

    2. DABor AEC can be used with HRP. I would recommend one ofthe following products :
    > IHC detectionkits 'EXPOSE': http://www.abcam.com/expose (see the 6 first refer...

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    1. yes,you need a secondary antibody such as ab97239 (Goat anti-Mouse IgG1 heavy chain (FITC) secondary antibody). The reason isthat the primary antibodies you want to use (ab25345, ab25346 andab25347) are not conjugated.

    2. a.Yes, ab98693 (G...

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    Yes,the chosen antibodies are suitable for Flow Cytometry and chicken samples.

    No, none of the chosen primary antibodies (ab25345, ab25346, ab25347) are conjugated, so you need a secondary antibody such as ab97239.

    The secondary ant...

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    Thank you for contacting us.

    I am happy to confirm that your chosen antibodies are suitable in Flow Cytometry and chicken, and that nothing else should be required.

    However, I am wondering about the usage of the ab37382 Chicken IgY...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"