The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 0.2µg for 106 cells.
FuncS: Use at an assay dependent dilution.
ab25423 can be used for in ovo depletion of gd T cells.
IHC-Fr: Use at an assay dependent dilution.
Note: samples need to be acetone fixed.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceT cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one a and one b chain or one d and one g chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level.
The g/d TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most g/d T cells are CD4-/CD8-, some are CD8+. T cells expressing the g/d TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease.
Cellular localizationType I membrane protein
T cell antigen receptor delta polypeptide antibody
T cell antigen receptor gamma polypeptide antibody
T cell rearranging gene gamma antibody
T cell receptor delta antibody
T cell receptor delta locus antibody
T cell receptor gamma antibody
T cell receptor gamma chain C region PT gamma 1/2 antibody
T cell receptor gamma cluster antibody
T cell receptor gamma locus antibody
T cell receptor T3 delta chain antibody
T cell receptor, delta (V,D,J,C) antibody
T cell receptors antibody
T cell surface glycoprotein CD3 delta chain antibody