Anti-TCR gamma + TCR delta antibody [UC7-13D5] (ab25252)
Key features and details
- Armenian Hamster monoclonal [UC7-13D5] to TCR gamma + TCR delta
- Suitable for: Flow Cyt, ICC, IP, IHC-Fr
- Reacts with: Mouse
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-TCR gamma + TCR delta antibody [UC7-13D5]
See all TCR gamma + TCR delta primary antibodies -
Description
Armenian Hamster monoclonal [UC7-13D5] to TCR gamma + TCR delta -
Host species
Armenian hamster -
Specificity
This antibody does not react with alpha-beta TCR expressing cells. -
Tested applications
Suitable for: Flow Cyt, ICC, IP, IHC-Frmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to TCR gamma + TCR delta. Mouse T cell clone G8
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General notes
This antibody has been shown to be useful in studies of in vitro activation of gamma-delta TCR bearing cells and in vitro depletion of gamma-delta TCR expressing cells.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading...
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Purity
Affinity purified -
Primary antibody notes
This antibody has been shown to be useful in studies of in vitro activation of gamma-delta TCR bearing cells and in vitro depletion of gamma-delta TCR expressing cells. -
Clonality
Monoclonal -
Clone number
UC7-13D5 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 1µg for 106 cells.
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ICC |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use 1µg for 106 cells. |
ICC
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Relevance
T cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one a and one b chain or one d and one g chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. The g/d TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most g/d T cells are CD4-/CD8-, some are CD8+. T cells expressing the g/d TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. -
Cellular localization
Type I membrane protein -
Alternative names
- CD_antigen=CD3d antibody
- CD3D antibody
- CD3G antibody
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Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab25252 has not yet been referenced specifically in any publications.