Overview

  • Product nameAnti-TCR gamma + TCR delta antibody [V65]
    See all TCR gamma + TCR delta primary antibodies
  • Description
    Mouse monoclonal [V65] to TCR gamma + TCR delta
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    CD3+ ve TCR alpha/beta -ve T cell hybridoma line (Rat).

  • General notes


    This antibody recognises a constant determinant of the rat gamma/delta T cell receptor. This antibody also stains all rat gamma/delta T cells, and also identifies as gamma/delta T cells a population of dendritic epidermal T lymphocytes. It may be used to stimulate the proliferation of gamma/delta T cells in the presence of either IL-2 or IL-4.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesThis antibody recognises a constant determinant of the rat gamma/delta T cell receptor. This antibody also stains all rat gamma/delta T cells, and also identifies as gamma/delta T cells a population of dendritic epidermal T lymphocytes. It may be used to stimulate the proliferation of gamma/delta T cells in the presence of either IL-2 or IL-4.
  • ClonalityMonoclonal
  • Clone numberV65
  • Myelomax63-Ag8.653
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells.





ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • RelevanceT cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one a and one b chain or one d and one g chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. The g/d TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most g/d T cells are CD4-/CD8-, some are CD8+. T cells expressing the g/d TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease.
  • Cellular localizationType I membrane protein
  • Database links
  • Alternative names
    • CD_antigen=CD3d antibody
    • CD3D antibody
    • CD3G antibody
    • T cell antigen receptor delta polypeptide antibody
    • T cell antigen receptor gamma polypeptide antibody
    • T cell rearranging gene gamma antibody
    • T cell receptor delta antibody
    • T cell receptor delta locus antibody
    • T cell receptor gamma antibody
    • T cell receptor gamma chain C region PT gamma 1/2 antibody
    • T cell receptor gamma cluster antibody
    • T cell receptor gamma locus antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor, delta (V,D,J,C) antibody
    • T cell receptors antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3D antibody
    • T3G antibody
    • TCRD antibody
    • TCRDV1 antibody
    • TCRG antibody
    • TRD antibody
    • TRD locus antibody
    • TRD@ antibody
    • TRG antibody
    • TRG locus antibody
    • TRGC2 antibody
    see all

References for Anti-TCR gamma + TCR delta antibody [V65] (ab23902)

This product has been referenced in:
  • Kühnlein P  et al. Gamma/delta T cells in fetal, neonatal, and adult rat lymphoid organs. Dev Immunol 4:181-8 (1995). Read more (PubMed: 8770557) »
  • Kühnlein P  et al. Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J Immunol 153:979-86 (1994). Read more (PubMed: 8027567) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"