Overview

  • Product name
    Anti-TCR V beta 10 antibody [G101]
  • Description
    Mouse monoclonal [G101] to TCR V beta 10
  • Specificity
    Approximately 4.2% of unseparated LEW lymph node cells were detected by this clone. Reactivity is restricted to alpha/beta T cells, of which 6.7% labelled.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Tissue/ cell preparation (Rat): T cell clone expressing the TCR V beta 10 gene product

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    ab23348 has been purified by ion exchange chromatography from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    G101
  • Myeloma
    P3x63-Ag8.653
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 10µl for 106 cells in 100µl, or 1/50 - 1/100.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments and one J segment of the beta locus are known to be incapable of encoding a protein and are considered pseudogenes. The beta locus also includes eight trypsinogen genes, three of which encode functional proteins and five of which are pseudogenes. Chromosomal abnormalities involving the T-cell receptor beta locus have been associated with T-cell lymphomas.
    • Cellular localization
      Plasma membrane
    • Database links

    References for Anti-TCR V beta 10 antibody [G101] (ab23348)

    This product has been referenced in:
    • Torres-Nagel NE  et al. Identification of rat Tcrb-V8.2, 8.5, and 10 gene products by monoclonal antibodies. Immunogenetics 37:305-8 (1993). Read more (PubMed: 8420840) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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