• Product nameAnti-TCR V beta 5 antibody [MEM-262]
  • Description
    Mouse monoclonal [MEM-262] to TCR V beta 5
  • SpecificityThe antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
  • Tested applicationsSuitable for: Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human thymoma cell line HPB-ALL.

  • General notesFunctional Application Activates T cells (V beta 5-related subset).


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from hybridoma culture supernatant by protein A affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberMEM-262
  • IsotypeIgG2a
  • Research areas


Our Abpromise guarantee covers the use of ab1898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceT cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. The Vß5 TCR is a variant of the TCR ß chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vß5 has been reported to recognize some staphylococcus bacterial superantigens.
    • Cellular localizationCell Membrane
    • Database links
    • Alternative names
      • T cell receptor antibody
      • TCB antibody
      • TCRB antibody
      • TRB antibody
      • TRB@ antibody
      see all

    References for Anti-TCR V beta 5 antibody [MEM-262] (ab1898)

    This product has been referenced in:
    • Pavlistová D  et al. A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor. Immunol Lett 88:105-8 (2003). Read more (PubMed: 12880678) »

    See 1 Publication for this product

    Product Wall

    Thank you for your patience. For ab9219, the originator has recommended the following. Since the antibody recognizes human cytokeratin 18 we recommend using the PBS-insoluble fraction (pellet) of human cell lines containing cytokeratin 18 (for example...

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    Thank you for your enquiry. The originator of ab1898 was able to provide me with the following information. Western blot detection: -material: HPB-ALL cell line -non-reducing conditions -detects only free beta chains -molecular weight 36 kDa ...

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