SpecificityThe antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceT cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments.
The Vß5 TCR is a variant of the TCR ß chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vß5 has been reported to recognize some staphylococcus bacterial superantigens.