Overview

  • Product name
    Anti-TCR V delta 2 antibody [15D]
  • Description
    Mouse monoclonal [15D] to TCR V delta 2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Cellular Activation, Blocking, IP, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Drosophila melanogaster, Non human primates, Rhesus monkey
  • Immunogen

    Full length protein corresponding to Human TCR V delta 2.
    Database link: 6964

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: 99% PBS, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    15D
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1 - 1/100.
Cellular Activation Use at an assay dependent concentration.

T-Cell Activation

Blocking Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

2 μg/Test

 

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Western blot analysis of TCR V delta 2 was performed by loading 25 ug of Jurkat (lane 1) and CEM (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a TCR V delta 2 monoclonal antibody (ab171103) at a dilution of 1:10 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using ECL Plus.Results show a band at ~19 kDa.

  • Flow cytometry analysis of TCR V delta 2 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR V delta 2 monoclonal antibody (ab171103) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

References

This product has been referenced in:
  • Yin S  et al. Hyperactivation and in situ recruitment of inflammatory Vd2 T cells contributes to disease pathogenesis in systemic lupus erythematosus. Sci Rep 5:14432 (2015). Read more (PubMed: 26395317) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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