Anti-TCR V gamma 9 antibody [7A5] (ab171109)

Overview

  • Product name
    Anti-TCR V gamma 9 antibody [7A5]
  • Description
    Mouse monoclonal [7A5] to TCR V gamma 9
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Cellular Activation, ELISA, IP, IHC-Fr, Neutralising, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Drosophila melanogaster, Non human primates
  • Immunogen

    Full length protein corresponding to Human TCR V gamma 9.
    Database link: 6965

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: 99% PBS, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    7A5
  • Isotype
    IgG1

Applications

Our Abpromise guarantee covers the use of ab171109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1 - 1/100.
Cellular Activation Use at an assay dependent concentration.

T-Cell Activation

ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

2 μg/Test

 

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Database links
  • Alternative names
    • T cell receptor gamma locus antibody
    • T-cell antigen receptor, gamma polypeptide antibody
    • T-cell rearranging gene, gamma antibody
    • T-cell receptor, gamma cluster antibody
    • TCRG antibody
    • TRG antibody
    see all

Images

  • Flow cytometry analysis of TCR V gamma 9 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR V gamma 9 monoclonal antibody (ab171109) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis

  • Western blot analysis of TCR V gamma 9 was performed by loading 25 ug of CEM cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a TCR V gamma 9 monoclonal antibody (ab171109) at a dilution of 1:10 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using an ECL Plus Western Blotting Substrate.Results show a band at ~18-28 kDa.

References

ab171109 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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