Overview

  • Product nameAnti-TCTP antibody
    See all TCTP primary antibodies
  • Description
    Rabbit polyclonal to TCTP
  • Specificityab65015 detects endogenous levels of total TCTP protein.
  • Tested applicationsSuitable for: ELISA, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human TCTP.

  • Positive control
    • Human breast carcinoma tissue. Extracts from HepG2, COLO205 and HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab65015 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab65015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • FunctionInvolved in calcium binding and microtubule stabilization.
  • Tissue specificityFound in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate.
  • Sequence similaritiesBelongs to the TCTP family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ27337 antibody
    • Fortilin antibody
    • Histamine releasing factor antibody
    • Histamine-releasing factor antibody
    • HRF antibody
    • Immunoglobulin E-dependent antibody
    • p02 antibody
    • p23 antibody
    • TCTP antibody
    • TCTP_HUMAN antibody
    • tpt1 antibody
    • Translationally controlled tumor protein antibody
    • Translationally-controlled tumor protein antibody
    • Tumor protein translationally controlled 1 antibody
    • tumor protein, translationally-controlled 1 antibody
    see all

Anti-TCTP antibody images

  • ab65015, at 1/50 - 1/100 dilution, staining TCTP in human breast carcinoma by Immunohistochemistry, using paraffin-embedded tissue, in the absence (left panel) or presence (right panel) of the immunising peptide. Antigen retrieval performed by boiling in a pressure cooker for 2-3 min. A polymer system was used for signal enhancing. Stained using DAB.
  • All lanes : Anti-TCTP antibody (ab65015) at 1/500 dilution

    Lane 1 : HepG2 cell extract (5-30 ug total protein)
    Lane 2 : COLO205 cell extract (5-30 ug total protein)
    Lane 3 : HeLa cell extract (5-30 ug total protein)
    Lane 4 : HeLa cell extract with immunising peptide (5-10 ug)


    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • ICC/IF image of ab65015 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65015, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TCTP antibody (ab65015)

ab65015 has not yet been referenced specifically in any publications.

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We have four polyclonal anti TCTP antibodies in our catalog, three are currently tested and guaranteed for WB.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"