Anti-TDH antibody - BSA and Azide free (ab188540)

Overview

  • Product name
    Anti-TDH antibody - BSA and Azide free
    See all TDH primary antibodies
  • Description
    Rabbit polyclonal to TDH - BSA and Azide free
  • Specificity
    ab188540 reacts with TRH toxin and TDH toxin of V. parahemolyticus.
  • Tested applications
    Suitable for: IP, WB, ELISAmore details
  • Immunogen

    Full length native protein (purified) corresponding to TDH. (TRH toxoid and toxin purified from culture medium of V. parahemolyticus TRH+ strain).
    Database link: BAB13778.1

  • Positive control
    • Condensed culture medium of V. parahaemolyticus (TRH+) .

Properties

Applications

Our Abpromise guarantee covers the use of ab188540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/2000 - 1/10000. Predicted molecular weight: 21 kDa.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    TDH (L threonine dehydrogenase) belongs to the sugar epimerase family and catalyzes the first of two steps in one of two L-threonine degradation pathways. According to reports, the human TDH gene is an expressed pseudogene encoding non-functional truncated proteins that are unable to make appropriate contacts with the substrates, L-threonine and NAD+.
  • Cellular localization
    Mitochondrial
  • Alternative names
    • FLJ25033 antibody
    • Inactive L threonine 3 dehydrogenase mitochondrial antibody
    • L threonine dehydrogenase antibody
    • SDR14E1P antibody

Images



  • Predicted band size : 21 kDa

    Lane 1: SDS-PAGE analysis of TRH toxin in ten-times condensed culture medium of V. parahaemolyticus (TRH+). Stained with CBB.

    Lane 2: WB analysis of TRH toxin in ten-times condensed culture medium of V. parahaemolyticus (TRH+) using ab188540 at 1/2000 dilution

  • Immunoprecipitation of TRH toxin from Vibrio parahaemolyticus (TRH+) culture medium with ab188540.
    Lane 1: TRH was precipitated with ab188540 from ten-times condensed culture medium of V. parahaemolyticus (TRH+), analysed by SDS-PAGE and stained with CBB.
    Lane 2: TRH was precipitated with ab188540 from ten-times condensed culture medium of V. parahaemolyticus (TRH+) and TRH was detected by WB with ab188540.
    Arrow indicates TRH band. H and L indicate heavy and light chain of IgG, respectively.

  • All lanes : Anti-TDH antibody - BSA and Azide free (ab188540) at 1/2000 dilution

    Lane 1 : Partially purified TDH toxin
    Lane 2 : Condensed culture medim of V. haemolyticus


    Predicted band size : 21 kDa

References

ab188540 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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