Overview

  • Product name
    Anti-TDP1 antibody [AT1F2]
    See all TDP1 primary antibodies
  • Description
    Mouse monoclonal [AT1F2] to TDP1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to the N terminal amino acids 1-298 of Human TDP1, purified from E. coli.

  • Positive control
    • MCF7 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    AT1F2
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 68 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Function
    DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.
  • Tissue specificity
    Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus.
  • Involvement in disease
    Spinocerebellar ataxia, autosomal recessive, with axonal neuropathy
  • Sequence similarities
    Belongs to the tyrosyl-DNA phosphodiesterase family.
  • Post-translational
    modifications
    Phosphorylated on serine and/or threonine residues, but not on tyrosine residues.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI838772 antibody
    • AW493413 antibody
    • FLJ11090 antibody
    • MGC104252 antibody
    • MGC112732 antibody
    • RP24-311F12.2 antibody
    • SCAN1 antibody
    • TDP1 antibody
    • TYDP antibody
    • TYDP1_HUMAN antibody
    • Tyr-DNA phosphodiesterase 1 antibody
    • Tyrosyl-DNA phosphodiesterase 1 antibody
    see all

Images

  • Anti-TDP1 antibody [AT1F2] (ab91035) at 1/1000 dilution + MCF7 cell lysate at 30 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 68 kDa

References

ab91035 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab91035.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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