Overview

  • Product name
  • Description
    Rabbit polyclonal to TDP43
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Recombinant fragment within Human TDP43 aa 178-393. The exact sequence is proprietary.
    Database link: Q13148

  • Positive control
    • Rat brain tissue; Mouse brain whole cell lysate; HeLa and H1299 whole cell lysates; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab183752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
  • Tissue specificity
    Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
  • Involvement in disease
    Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
  • Sequence similarities
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain
    The RRM domains can bind to both DNA and RNA.
  • Post-translational
    modifications
    Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
  • Cellular localization
    Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALS10 antibody
    • OTTHUMP00000002171 antibody
    • OTTHUMP00000002172 antibody
    • OTTHUMP00000002173 antibody
    • TADBP_HUMAN antibody
    • TAR DNA binding protein 43 antibody
    • TAR DNA binding protein antibody
    • TAR DNA-binding protein 43 antibody
    • TARDBP antibody
    • TDP 43 antibody
    • TDP-43 antibody
    • TDP43 antibody
    see all

Images

  • Anti-TDP43 antibody (ab183752) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 44 kDa



    10% SDS PAGE

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling TARDBP with ab183752 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled Red.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling TARDBP with ab183752 at 1/500 dilution.

  • All lanes : Anti-TDP43 antibody (ab183752) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate mock control
    Lane 2 : HeLa whole cell lysate transfected with shTDP-43

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 44 kDa



    10% SDS PAGE

  • Anti-TDP43 antibody (ab183752) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 44 kDa



    10% SDS PAGE

References

ab183752 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183752.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up