Overview

  • Product name
  • Description
    Goat polyclonal to TDP43
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human TDP43 aa 256-269 (internal sequence) (Cysteine residue).
    Sequence:

    C-HISNAEPKHNSNRQ

  • Positive control
    • Human lysates of cell line Jurkat

Properties

Applications

Our Abpromise guarantee covers the use of ab80608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 45 kDa.

Target

  • Function
    DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
  • Tissue specificity
    Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
  • Involvement in disease
    Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
  • Sequence similarities
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain
    The RRM domains can bind to both DNA and RNA.
  • Post-translational
    modifications
    Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
  • Cellular localization
    Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALS10 antibody
    • OTTHUMP00000002171 antibody
    • OTTHUMP00000002172 antibody
    • OTTHUMP00000002173 antibody
    • TADBP_HUMAN antibody
    • TAR DNA binding protein 43 antibody
    • TAR DNA binding protein antibody
    • TAR DNA-binding protein 43 antibody
    • TARDBP antibody
    • TDP 43 antibody
    • TDP-43 antibody
    • TDP43 antibody
    see all

Anti-TDP43 antibody images

  • All lanes : Anti-TDP43 antibody (ab80608) at 1 µg/ml

    Lane 1 : Human cerebellum cell lysate
    Lane 2 : Human frontal cotex cell lysate
    Lane 3 : Human hippocampus cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 45 kDa
  • Anti-TDP43 antibody (ab80608) at 0.3 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

References for Anti-TDP43 antibody (ab80608)

ab80608 has not yet been referenced specifically in any publications.

Product Wall

Application
ELISA
Sample
Human Recombinant protein (Full length produced in E.coli, His-tag purified)
Specification
Full length produced in E.coli, His-tag purified
Type
Sandwich (Capture)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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Submitted Aug 06 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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