1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionScaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins.
Tissue specificityDetected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling TDRD3 with ab95879 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - TDRD3 antibody (ab95879)
All lanes : Anti-TDRD3 antibody (ab95879) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size : 73 kDa
Exposure time : 3 minutes
Immunoprecipitation - TDRD3 antibody (ab95879)
Detection of TDRD3 by Western Blot of Immunprecipitate.
ab95879 at 0.4µg/ml staining TDRD3 in HeLa whole cell lysate immunoprecipitated using ab95879 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-TDRD3 antibody (ab95879)
has not yet been referenced specifically in any publications.
Publishing research using ab95879? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"