Overview

  • Product nameAnti-TDRD7 antibody
    See all TDRD7 primary antibodies
  • Description
    Rabbit polyclonal to TDRD7
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Dog, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human TDRD7.

  • Positive control
    • This antibody gave a positive signal in both Human testis and lung tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab103866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 124 kDa (predicted molecular weight: 124 kDa).

Target

  • Sequence similaritiesContains 2 Tudor domains.
  • Cellular localizationCytoplasm. Present in chromatoid body (CB) of spermatids (mammalian counterpart of germplasm, pole plasm or polar granules in Drosophila germ cells), also named processing bodies (P-bodies) in somatic cells. Detected in the multilobular cytoplasmic CBs (also called intermitochondrial cementin) in pachytene spermatocytes and as a single perinuclear CB in haploid round spermatids.
  • Information by UniProt
  • Database links
  • FormFound in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. Found in a complex containing CABLES1, CDK16 and CDK17. Interacts with CABLES1, CDK17 and PIWIL1.
  • Alternative names
    • CATC4 antibody
    • KIAA1529 antibody
    • PCTAIRE 2 antibody
    • PCTAIRE2 binding protein antibody
    • PCTAIRE2 BP antibody
    • PCTAIRE2-binding protein antibody
    • PCTAIRE2BP antibody
    • RP11-508D10.1 antibody
    • TDRD 7 antibody
    • Tdrd7 antibody
    • TDRD7_HUMAN antibody
    • TrAP antibody
    • Tudor domain containing 7 antibody
    • Tudor domain-containing protein 7 antibody
    • Tudor repeat associator with PCTAIRE 2 antibody
    see all

Anti-TDRD7 antibody images

  • All lanes : Anti-TDRD7 antibody (ab103866) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 124 kDa
    Observed band size : 124 kDa
    Additional bands at : 37 kDa,89 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-TDRD7 antibody (ab103866)

ab103866 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Bos taurus Tissue lysate - whole (ovary, testis and oocytes)
Gel Running Conditions Reduced Denaturing (gel 8%)
Loading amount 25 µg
Specification ovary, testis and oocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Feb 09 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (gel 8%)
Sample Human Purified protein (Periodontal ligament cell-conditioned medium)
Specification Periodontal ligament cell-conditioned medium
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Oct 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"