Overview

  • Product nameAnti-TDRD7 antibody
    See all TDRD7 primary antibodies
  • Description
    Rabbit polyclonal to TDRD7
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 807-835 of Human TDRD7 (NP_055105.2).

  • Positive control
    • A549 cell lysate; Mouse bladder tissue lysate; Human brain tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: CACO-2.

Properties

Applications

Our Abpromise guarantee covers the use of ab107675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 124 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similaritiesContains 2 Tudor domains.
  • Cellular localizationCytoplasm. Present in chromatoid body (CB) of spermatids (mammalian counterpart of germplasm, pole plasm or polar granules in Drosophila germ cells), also named processing bodies (P-bodies) in somatic cells. Detected in the multilobular cytoplasmic CBs (also called intermitochondrial cementin) in pachytene spermatocytes and as a single perinuclear CB in haploid round spermatids.
  • Information by UniProt
  • Database links
  • FormFound in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. Found in a complex containing CABLES1, CDK16 and CDK17. Interacts with CABLES1, CDK17 and PIWIL1.
  • Alternative names
    • CATC4 antibody
    • KIAA1529 antibody
    • PCTAIRE 2 antibody
    • PCTAIRE2 binding protein antibody
    • PCTAIRE2 BP antibody
    • PCTAIRE2-binding protein antibody
    • PCTAIRE2BP antibody
    • RP11-508D10.1 antibody
    • TDRD 7 antibody
    • Tdrd7 antibody
    • TDRD7_HUMAN antibody
    • TrAP antibody
    • Tudor domain containing 7 antibody
    • Tudor domain-containing protein 7 antibody
    • Tudor repeat associator with PCTAIRE 2 antibody
    see all

Anti-TDRD7 antibody images

  • ICC/IF image of ab107675 stained CACO-2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107675 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-TDRD7 antibody (ab107675) at 1/100 dilution + A549 cell lysate at 35 µg

    Predicted band size : 124 kDa
  • Anti-TDRD7 antibody (ab107675) at 1/100 dilution + Mouse bladder tissue lysate at 35 µg

    Predicted band size : 124 kDa
  • ab107675, at 1/50 dilution, staining TDRD7 in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

References for Anti-TDRD7 antibody (ab107675)

ab107675 has not yet been referenced specifically in any publications.

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