- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-TdT antibody
See all TdT primary antibodies -
DescriptionRabbit polyclonal to TdT
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Host speciesRabbit
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Tested applicationsSuitable for: IP, WB, IHC-P, IHC-Frmore details
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Species reactivityReacts with: Rat, Human
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Immunogen
Synthetic cyclic peptide (common to human terminal deoxynuleotidyl transferase (TdT) protein.
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Positive control
- Tested with bone marrow cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab14772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/250. | |
| WB | 1/1000. Detects a band of approximately 58 kDa. | |
| IHC-P | 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-Fr | 1/250. |
Target
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FunctionTemplate-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
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Sequence similaritiesBelongs to the DNA polymerase type-X family.
Contains 1 BRCT domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 1791 Human
- Entrez Gene: 294051 Rat
- Omim: 187410 Human
- SwissProt: P04053 Human
- Unigene: 534206 Human
- Unigene: 143741 Rat
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Alternative names
- Deoxynucleotidyltransferase terminal antibody
- DNA nucleotidylexotransferase antibody
- DNTT antibody
see all
References
This product has been referenced in:
- Boubakour-Azzouz I et al. Terminal deoxynucleotidyl transferase requires KU80 and XRCC4 to promote N-addition at non-V(D)J chromosomal breaks in non-lymphoid cells. Nucleic Acids Res 40:8381-91 (2012). WB, ICC/IF . Read more (PubMed: 22740656) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"