Overview

  • Product name
  • Description
    Rabbit polyclonal to TdT
  • Tested applications
    Suitable for: IP, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic cyclic peptide (common to human terminal deoxynuleotidyl transferase (TdT) protein.

  • Positive control
    • Tested with bone marrow cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/250.
WB 1/1000. Detects a band of approximately 58 kDa.
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/250.

Target

  • Function
    Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
  • Sequence similarities
    Belongs to the DNA polymerase type-X family.
    Contains 1 BRCT domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deoxynucleotidyltransferase terminal antibody
    • DNA nucleotidylexotransferase antibody
    • DNTT antibody
    • Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
    • TDT antibody
    • TDT_HUMAN antibody
    • Terminal addition enzyme antibody
    • Terminal deoxynucleotidyltransferase antibody
    • Terminal deoxyribonucleotidyltransferase antibody
    • Terminal transferase antibody
    see all

References

This product has been referenced in:
  • Boubakour-Azzouz I  et al. Terminal deoxynucleotidyl transferase requires KU80 and XRCC4 to promote N-addition at non-V(D)J chromosomal breaks in non-lymphoid cells. Nucleic Acids Res 40:8381-91 (2012). WB, ICC/IF . Read more (PubMed: 22740656) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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