Recombinant
RabMAb

Anti-TdT antibody [EPR2975] (ab79395)

Overview

  • Product name
    Anti-TdT antibody [EPR2975]
    See all TdT primary antibodies
  • Description
    Rabbit monoclonal [EPR2975] to TdT
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in human TdT.

  • Positive control
    • Fetal thymus and Jurkat cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR2975
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab79395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
    • Sequence similarities
      Belongs to the DNA polymerase type-X family.
      Contains 1 BRCT domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deoxynucleotidyltransferase terminal antibody
      • DNA nucleotidylexotransferase antibody
      • DNTT antibody
      • Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
      • TDT antibody
      • TDT_HUMAN antibody
      • Terminal addition enzyme antibody
      • Terminal deoxynucleotidyltransferase antibody
      • Terminal deoxyribonucleotidyltransferase antibody
      • Terminal transferase antibody
      see all

    Images

    • All lanes : Anti-TdT antibody [EPR2975] (ab79395) at 1/2000 dilution

      Lane 1 : Fetal thymus cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 58 kDa
      Observed band size : 58 kDa

    References

    ab79395 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
    Sample
    Mouse Cell (B cell (lymphocytes))
    Specification
    B cell (lymphocytes)
    Permeabilization
    Yes - 0.5% triton
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 23 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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