Overview

  • Product name
    Anti-TdT antibody [EPR2976Y]
    See all TdT primary antibodies
  • Description
    Rabbit monoclonal [EPR2976Y] to TdT
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C terminal of human TdT

  • Positive control
    • Human thymus and Jurkat lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR2976Y
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab76544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IP 1/20.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
    • Sequence similarities
      Belongs to the DNA polymerase type-X family.
      Contains 1 BRCT domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deoxynucleotidyltransferase terminal antibody
      • DNA nucleotidylexotransferase antibody
      • DNTT antibody
      • Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
      • TDT antibody
      • TDT_HUMAN antibody
      • Terminal addition enzyme antibody
      • Terminal deoxynucleotidyltransferase antibody
      • Terminal deoxyribonucleotidyltransferase antibody
      • Terminal transferase antibody
      see all

    Images

    • All lanes : Anti-TdT antibody [EPR2976Y] (ab76544) at 1/10000 dilution

      Lane 1 : Human thymus lysate
      Lane 2 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 58 kDa
      Observed band size : 58 kDa
      Additional bands at : 24 kDa,35 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:
    • Aït Ghezali L  et al. Induction of endoplasmic reticulum calcium pump expression during early leukemic B cell differentiation. J Exp Clin Cancer Res 36:87 (2017). Read more (PubMed: 28651627) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab76544.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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