The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
1/100 - 1/250.
Application notesIs unsuitable for Flow Cyt or IHC-P.
FunctionTemplate-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Sequence similaritiesBelongs to the DNA polymerase type-X family. Contains 1 BRCT domain.