The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/10 - 1/50 in an ABC method.
Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. Optimal incubation conditions should be determined by the user.
Paraffin-embedded tissue sections require heat mediated antigen retrieval using 10 mM citrate buffer, pH 6.0. prior to immunostaining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTemplate-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Sequence similaritiesBelongs to the DNA polymerase type-X family. Contains 1 BRCT domain.