Anti-TEA domain family member 2 antibody [404C5a] (ab54374)

Overview

  • Product name
    Anti-TEA domain family member 2 antibody [404C5a]
    See all TEA domain family member 2 primary antibodies
  • Description
    Mouse monoclonal [404C5a] to TEA domain family member 2
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    TEA domain family member 2 recombinant fragment (Human)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab54374 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    404C5a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 49 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Function
    Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.
  • Sequence similarities
    Contains 1 TEA DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Embryonic TEA domain containing factor antibody
    • Etdf antibody
    • ETEF 1 antibody
    • ETF antibody
    • TEA domain family member 2 antibody
    • TEAD 2 antibody
    • TEAD-2 antibody
    • TEAD2 antibody
    • Tead2 protein (TEA domain family member 2 (Mapped), isoform CRA_b) antibody
    • TEAD2_HUMAN antibody
    • TEF 4 antibody
    • TEF4 antibody
    • Transcriptional enhancer factor 4 antibody
    • Transcriptional enhancer factor TEF 4 antibody
    • Transcriptional enhancer factor TEF-4 antibody
    see all

Images

References

ab54374 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Jul 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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