Anti-TEA domain family member 2 antibody (ab110783)

Overview

  • Product name
    Anti-TEA domain family member 2 antibody
    See all TEA domain family member 2 primary antibodies
  • Description
    Rabbit polyclonal to TEA domain family member 2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse TEA domain family member 2 aa 200-300 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P48301

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Brain 0 day; Mouse Cerebellum; Mouse Hippocampus; Mouse Testis.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 49 kDa).

Target

  • Function
    Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.
  • Sequence similarities
    Contains 1 TEA DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Embryonic TEA domain containing factor antibody
    • Etdf antibody
    • ETEF 1 antibody
    • ETF antibody
    • TEA domain family member 2 antibody
    • TEAD 2 antibody
    • TEAD-2 antibody
    • TEAD2 antibody
    • Tead2 protein (TEA domain family member 2 (Mapped), isoform CRA_b) antibody
    • TEAD2_HUMAN antibody
    • TEF 4 antibody
    • TEF4 antibody
    • Transcriptional enhancer factor 4 antibody
    • Transcriptional enhancer factor TEF 4 antibody
    • Transcriptional enhancer factor TEF-4 antibody
    see all

Images

  • All lanes : Anti-TEA domain family member 2 antibody (ab110783) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 3 : Testis (Mouse) Tissue Lysate
    Lane 4 : Cerebellum Rat Tissue Lysate
    Lane 5 : Mouse Hippocampus Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 68 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab110783 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab110783 has not yet been referenced specifically in any publications.

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