Overview

  • Product nameAnti-TEAD3 antibody
    See all TEAD3 primary antibodies
  • Description
    Rabbit polyclonal to TEAD3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 81-110 of Human TEAD3 (NP_003205.2).

  • Positive control
    • NCI-H292 cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab123327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 49 kDa.

Target

  • FunctionTranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to multiple functional elements of the human chorionic somatomammotropin-B gene enhancer.
  • Tissue specificityPreferentially expressed in the placenta.
  • Sequence similaritiesContains 1 TEA DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DTEF 1 antibody
    • DTEF-1 antibody
    • DTEF1 antibody
    • ETFR 1 antibody
    • ETFR1 antibody
    • TEA domain family member 3 antibody
    • TEA domain family member 5 antibody
    • TEAD 3 antibody
    • TEAD-3 antibody
    • Tead3 antibody
    • TEAD3_HUMAN antibody
    • TEAD5 antibody
    • TEF 5 antibody
    • TEF5 antibody
    • Transcriptional enhancer factor 5 antibody
    • Transcriptional enhancer factor TEF 5 (DTEF 1) antibody
    • Transcriptional enhancer factor TEF 5 antibody
    • Transcriptional enhancer factor TEF-5 antibody
    see all

Anti-TEAD3 antibody images

  • All lanes : Anti-TEAD3 antibody (ab123327) at 1/250 dilution

    Lane 1 : Water
    Lane 2 : U373 cell lysate at 60 µg
    Lane 3 : Fruit fly (Drosophila melanogaster) whole larvae tissue lysate at 60 µg

    Secondary
    HRP-conjugated rabbit anti-rabbit IgG polyclonal at 1/30000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa


    Exposure time : 10 minutes

    This image is courtesy of an Abreview submitted by Kristin Eikmeier

    See Abreview

  • Anti-TEAD3 antibody (ab123327) at 1/100 dilution + NCI-H292 cell line lysates at 35 µg

    Predicted band size : 49 kDa

References for Anti-TEAD3 antibody (ab123327)

ab123327 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Denaturing (12 % SDS-Gel)
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (whole larvae tissue)
Specification whole larvae tissue
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Miss. Kristin Eikmeier

Verified customer

Submitted Mar 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"