Anti-TEAD4 antibody (ab155244)
Key features and details
- Rabbit polyclonal to TEAD4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TEAD4 antibody
See all TEAD4 primary antibodies -
Description
Rabbit polyclonal to TEAD4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Pig -
Immunogen
Recombinant fragment corresponding to a region within amino acids 126-416 of the Human TEAD4 protein (Q15561).
-
Positive control
- WB: HeLa whole cell lysate. ICC/IF: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
-
Function
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif. -
Tissue specificity
Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart. -
Sequence similarities
Contains 1 TEA DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 395542 Chicken
- Entrez Gene: 7004 Human
- Entrez Gene: 21679 Mouse
- Entrez Gene: 100152085 Pig
- Omim: 601714 Human
- SwissProt: P48984 Chicken
- SwissProt: Q15561 Human
- SwissProt: Q62296 Mouse
see all -
Alternative names
- EFTR 2 antibody
- EFTR2 antibody
- hRTEF 1B antibody
see all
Images
-
All lanes : Anti-TEAD4 antibody (ab155244) at 1/1000 dilution (Primary ab Dilution Anti-TEAD4 antibody)
All lanes :Anti-TEAD4 antibody (ab155244) with NFDM/TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 48 kDa
Exposure time: 180 seconds -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling TEAD4 with ab155244 at 1/200 in green and co-stained with Alpha-tubulin at 1:2000 in red.
-
Anti-TEAD4 antibody (ab155244) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 48 kDa
7.5% SDS PAGE
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab155244 has been referenced in 1 publication.
- Bai T et al. Establishment of human induced trophoblast stem-like cells from term villous cytotrophoblasts. Stem Cell Res 56:102507 (2021). PubMed: 34454392