Overview

  • Product nameAnti-TEAD4 antibody
    See all TEAD4 primary antibodies
  • Description
    Mouse monoclonal to TEAD4
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-362 of Human TEAD4

Properties

Applications

Our Abpromise guarantee covers the use of ab58308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionTranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.
  • Tissue specificityPreferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
  • Sequence similaritiesContains 1 TEA DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EFTR 2 antibody
    • EFTR2 antibody
    • hRTEF 1B antibody
    • hRTEF1B antibody
    • MGC9014 antibody
    • OTTHUMP00000238119 antibody
    • OTTHUMP00000238122 antibody
    • OTTHUMP00000238124 antibody
    • Related to TEF 1 antibody
    • Related to TEF1 antibody
    • Related transcription enhancer factor 1B antibody
    • RTEF1 antibody
    • TCF13L1 antibody
    • TEA domain family member 4 antibody
    • TEAD 4 antibody
    • TEAD-4 antibody
    • TEAD4 antibody
    • TEAD4_HUMAN antibody
    • TEF 3 antibody
    • TEF3 antibody
    • TEFR 1 antibody
    • TEFR1 antibody
    • Transcription factor 13 (SV40 transcriptional enhancer factor) like 1 antibody
    • Transcription factor 13 like 1 antibody
    • Transcription factor 13-like 1 antibody
    • Transcription factor RTEF 1 antibody
    • Transcription factor RTEF-1 antibody
    • Transcription factor RTEF1 antibody
    • Transcriptional enhancer factor 1 related antibody
    • Transcriptional enhancer factor 3 antibody
    • Transcriptional enhancer factor TEF 3 antibody
    • Transcriptional enhancer factor TEF-3 antibody
    • Transcriptional enhancer factor TEF3 antibody
    see all

Anti-TEAD4 antibody images

  • TEAD4 antibody (ab58308) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
  • Western blot against tagged recombinant protein immunogen using ab58308 TEAD4 antibody at 1ug/ml. Predicted band size of immunogen is 66 kDa
  • Anti-TEAD4 antibody (ab58308) + human spleen
  • Overlay histogram showing HeLa cells stained with ab58308 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58308, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-TEAD4 antibody (ab58308)

ab58308 has not yet been referenced specifically in any publications.

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