The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionTranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.
Tissue specificityPreferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TEAD4 with ab85358 at 1/250. Cells wew fixed with 4% PFA for 30 minutes and blocked with 5% goat serum + 1% BSA + 0.02% fish gelatin overnight at 4°C. Cells were incubated with the primary antibody overnight at 4°C. An Alexa Fluor 546-conjugated anti-rabbit IgG (1/500, room temperature, 2 hours) was used as the secondary antibody.
Western blot - TEAD4 antibody (ab85358)
Anti-TEAD4 antibody (ab85358) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution