• Product nameAnti-TEAD4 antibody
    See all TEAD4 primary antibodies
  • Description
    Rabbit polyclonal to TEAD4
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (LVKFWADLNT NIEDEGSSFY GVSSQYESPE NMIITCSTKV CSFGKQVVEK) of human TEAD4 (NP_958849).

  • Positive control
    • Transfected 293T cell lysate



Our Abpromise guarantee covers the use of ab85358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionTranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.
  • Tissue specificityPreferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
  • Sequence similaritiesContains 1 TEA DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EFTR 2 antibody
    • EFTR2 antibody
    • hRTEF 1B antibody
    • hRTEF1B antibody
    • MGC9014 antibody
    • OTTHUMP00000238119 antibody
    • OTTHUMP00000238122 antibody
    • OTTHUMP00000238124 antibody
    • Related to TEF 1 antibody
    • Related to TEF1 antibody
    • Related transcription enhancer factor 1B antibody
    • RTEF1 antibody
    • TCF13L1 antibody
    • TEA domain family member 4 antibody
    • TEAD 4 antibody
    • TEAD-4 antibody
    • TEAD4 antibody
    • TEAD4_HUMAN antibody
    • TEF 3 antibody
    • TEF3 antibody
    • TEFR 1 antibody
    • TEFR1 antibody
    • Transcription factor 13 (SV40 transcriptional enhancer factor) like 1 antibody
    • Transcription factor 13 like 1 antibody
    • Transcription factor 13-like 1 antibody
    • Transcription factor RTEF 1 antibody
    • Transcription factor RTEF-1 antibody
    • Transcription factor RTEF1 antibody
    • Transcriptional enhancer factor 1 related antibody
    • Transcriptional enhancer factor 3 antibody
    • Transcriptional enhancer factor TEF 3 antibody
    • Transcriptional enhancer factor TEF-3 antibody
    • Transcriptional enhancer factor TEF3 antibody
    see all

Anti-TEAD4 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TEAD4 with ab85358 at 1/250. Cells wew fixed with 4% PFA for 30 minutes and blocked with 5% goat serum + 1% BSA + 0.02% fish gelatin overnight at 4°C. Cells were incubated with the primary antibody overnight at 4°C. An Alexa Fluor 546-conjugated anti-rabbit IgG (1/500, room temperature, 2 hours) was used as the secondary antibody.

  • Anti-TEAD4 antibody (ab85358) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-TEAD4 antibody (ab85358)

ab85358 has not yet been referenced specifically in any publications.

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