Anti-TEAD4 antibody [5H3] (ab58310)
Key features and details
- Mouse monoclonal [5H3] to TEAD4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-TEAD4 antibody [5H3]
See all TEAD4 primary antibodies -
Description
Mouse monoclonal [5H3] to TEAD4 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to amino acids 151-261 of Human TEAD4
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General notes
This product was changed from ascites to tissue culture supernatant on 30th May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 8% Sodium chloride, 0.6% Dibasic monohydrogen sodium phosphate, 0.2% Monobasic dihydrogen potassium phosphate, 0.2% Potassium chloride, 91% Water -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5H3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa. |
Target
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Function
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif. -
Tissue specificity
Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart. -
Sequence similarities
Contains 1 TEA DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7004 Human
- Omim: 601714 Human
- SwissProt: Q15561 Human
- Unigene: 94865 Human
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Alternative names
- EFTR 2 antibody
- EFTR2 antibody
- hRTEF 1B antibody
see all
Images
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All lanes : Anti-TEAD4 antibody [5H3] (ab58310) at 1/1000 dilution
Lane 1 : TEAD1 Human Recombinant Protein
Lane 2 : TEAD2 Human Recombinant Protein
Lane 3 : TEAD3 Human Recombinant Protein
Lane 4 : TEAD4 Human Recombinant Protein
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG at 1/5000 dilution
Predicted band size: 48 kDa
Observed band size: 50-75 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-TEAD4 antibody [5H3] (ab58310) at 1 µg
Lane 1 : Wild-type A549 cell lysate
Lane 2 : TEAD4 CRISPR-Cas9 edited A549 cell lysate
Lane 3 : T-47D cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDaFalse colour image of Western blot: Anti-TEAD4 antibody [5H3] staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab58310 was shown to bind specifically to TEAD4. A band was observed at 48 kDa in wild-type A549 cell lysates with no signal observed at this size in TEAD4 CRISPR-Cas9 edited cell line ab277855. The band observed in the CRISPR-Cas9 edited lysate lane below 48 kDa is likely to represent a truncated form of TEAD4. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and TEAD4 CRISPR-Cas9 edited A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
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TEAD4 antibody (ab58310) at 1ug/lane + HeLa cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (80)
ab58310 has been referenced in 80 publications.
- Hwang JH et al. TAZ links exercise to mitochondrial biogenesis via mitochondrial transcription factor A. Nat Commun 13:653 (2022). PubMed: 35115527
- Wang J et al. Selective YAP activation in Procr cells is essential for ovarian stem/progenitor expansion and epithelium repair. Elife 11:N/A (2022). PubMed: 35285801
- Paim LMG & FitzHarris G Cell size and polarization determine cytokinesis furrow ingression dynamics in mouse embryos. Proc Natl Acad Sci U S A 119:e2119381119 (2022). PubMed: 35294282
- Hori N et al. Vestigial-like family member 3 stimulates cell motility by inducing high-mobility group AT-hook 2 expression in cancer cells. J Cell Mol Med 26:2686-2697 (2022). PubMed: 35366053
- Suzuki D et al. CDX2 downregulation in mouse mural trophectoderm during peri-implantation is heteronomous, dependent on the YAP-TEAD pathway and controlled by estrogen-induced factors. Reprod Med Biol 21:e12446 (2022). PubMed: 35386376