Overview

  • Product name
    Anti-TECK antibody (Biotin)
    See all TECK primary antibodies
  • Description
    Rabbit polyclonal to TECK (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Sandwich ELISA, WB, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human TECK.

Properties

  • Form
    Lyophilised:Centrifuge vial prior to opening. Reconstitute in 50 µ sterile PBS containing 0.1% BSA to give a concentration of 1.0 mg/ml.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab83146 is sterile filtered. ab83146 was purified by affinity chromatography and then biotinylated.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to TECK (ab2164).

Assuming 100µl/well, a concentration of 0.5-2.0 µg/ml of ab2164 will detect at least 0.2-0.4 ng/well of recombinant human TECK when used with ab83146 as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.

WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 17 kDa.
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml.

Target

  • Function
    Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1.
  • Tissue specificity
    Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A130072A22Rik antibody
    • C-C motif chemokine 25 antibody
    • CCL 25 antibody
    • CCL25 antibody
    • CCL25_HUMAN antibody
    • Chemokine (C C motif) ligand 25 antibody
    • Chemokine (CC motif) ligand 25 antibody
    • Chemokine C C motif ligand 25 antibody
    • Chemokine CC motif ligand 25 antibody
    • Chemokine TECK antibody
    • Ck beta 15 antibody
    • Ckb 15 antibody
    • Ckb15 antibody
    • MGC125074 antibody
    • MGC150327 antibody
    • SCY A25 antibody
    • SCYA 25 antibody
    • SCYA25 antibody
    • Small inducible cytokine A25 antibody
    • Small inducible cytokine A25 isoform 1 precursor antibody
    • Small inducible cytokine A25 isoform 2 precursor antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 25 antibody
    • Small inducible cytokine subfamily A Cys Cys member 25 antibody
    • Small inducible cytokine subfamily A, member 25 antibody
    • Small-inducible cytokine A25 antibody
    • TECK antibody
    • TECKvar antibody
    • Thymus expressed chemokine antibody
    • Thymus-expressed chemokine antibody
    see all

References

ab83146 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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