Overview

  • Product name
    Anti-TECK antibody (Biotin)
    See all TECK primary antibodies
  • Description
    Rabbit polyclonal to TECK (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Sandwich ELISA, WB, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human TECK.

Properties

  • Form
    Lyophilised:Centrifuge vial prior to opening. Reconstitute in 50 µ sterile PBS containing 0.1% BSA to give a concentration of 1.0 mg/ml.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab83146 is sterile filtered. ab83146 was purified by affinity chromatography and then biotinylated.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to TECK (ab2164).

Assuming 100µl/well, a concentration of 0.5-2.0 µg/ml of ab2164 will detect at least 0.2-0.4 ng/well of recombinant human TECK when used with ab83146 as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 17 kDa.
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml.

Target

  • Function
    Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1.
  • Tissue specificity
    Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • A130072A22Rik antibody
      • C-C motif chemokine 25 antibody
      • CCL 25 antibody
      • CCL25 antibody
      • CCL25_HUMAN antibody
      • Chemokine (C C motif) ligand 25 antibody
      • Chemokine (CC motif) ligand 25 antibody
      • Chemokine C C motif ligand 25 antibody
      • Chemokine CC motif ligand 25 antibody
      • Chemokine TECK antibody
      • Ck beta 15 antibody
      • Ckb 15 antibody
      • Ckb15 antibody
      • MGC125074 antibody
      • MGC150327 antibody
      • SCY A25 antibody
      • SCYA 25 antibody
      • SCYA25 antibody
      • Small inducible cytokine A25 antibody
      • Small inducible cytokine A25 isoform 1 precursor antibody
      • Small inducible cytokine A25 isoform 2 precursor antibody
      • Small inducible cytokine subfamily A (Cys Cys) member 25 antibody
      • Small inducible cytokine subfamily A Cys Cys member 25 antibody
      • Small inducible cytokine subfamily A, member 25 antibody
      • Small-inducible cytokine A25 antibody
      • TECK antibody
      • TECKvar antibody
      • Thymus expressed chemokine antibody
      • Thymus-expressed chemokine antibody
      see all

    References

    ab83146 has not yet been referenced specifically in any publications.

    Publishing research using ab83146? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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