The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 240 kDa.
Use at an assay dependent dilution.
Use at an assay dependent concentration.
FunctionOne of the major non-collagenous components of the tectorial membrane (By similarity). The tectorial membrane is an extracellular matrix of the inner ear that covers the neuroepithelium of the cochlea and contacts the stereocilia bundles of specialized sensory hair cells. Sound induces movement of these hair cells relative to the tectorial membrane, deflects the stereocilia and leads to fluctuations in hair-cell membrane potential, transducing sound into electrical signals.
Involvement in diseaseDefects in TECTA are the cause of deafness autosomal dominant type 12 (DFNA12) [MIM:601543]; also known as DFNA8. DFNA12 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Defects in TECTA are the cause of deafness autosomal recessive type 21 (DFNB21) [MIM:603629].
DomainZona pellucida domain may enable to form filaments.
Post-translational modificationsThe presence of a hydrophobic C-terminus preceded by a potential cleavage site strongly suggests that tectorins are synthesized as glycosylphosphatidylinositol-linked, membrane-bound precursors. Tectorins are targeted to the apical surface of the inner ear epithelia by the lipid and proteolytically released into the extracellular compartment.
Cellular localizationCell membrane. Secreted > extracellular space > extracellular matrix. Found in the non-collagenous matrix of the tectorial membrane.