Overview

  • Product nameAnti-TEKT2 antibody
    See all TEKT2 primary antibodies
  • Description
    Mouse monoclonal to TEKT2
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Partial recombinant fragment: MATLSVKPSR RFQLPDWHTN SYLLSTNAQL QRDASHQIRQ EARVLRNETN NQTIWDEHDN RTRLVERIDT VNRWKEMLDK CLTDLDAEID ALTQMKESA, corresponding to amino acids 1-99 of TEKT2, with a tag

Properties

Applications

Our Abpromise guarantee covers the use of ab60918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 50 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionStructural component of ciliary and flagellar microtubules. Plays a key role in the assembly or attachment of the inner dynein arm to microtubules in sperm flagella and tracheal cilia. Forms filamentous polymers in the walls of ciliary and flagellar microtubules.
  • Tissue specificityExpressed at high levels in testis, trachea and fetal lung, and at lower levels in ovary, pituitary, adult lung, fetal brain and fetal kidney.
  • Sequence similaritiesBelongs to the tektin family.
  • Post-translational
    modifications
    Tyrosine phosphorylated.
  • Cellular localizationCell projection > cilium > flagellum. Cytoplasm > cytoskeleton > cilium axoneme. Cytoplasm > cytoskeleton > flagellum axoneme. In sperm, observed in a discontinuous punctate pattern in the flagellum and in the postacrosomal head region.
  • Information by UniProt
  • Database links
  • Alternative names
    • TEKT2 antibody
    • TEKT2_HUMAN antibody
    • TEKTB1 antibody
    • Tektin 2 antibody
    • Tektin B1 antibody
    • Tektin t antibody
    • Tektin-2 antibody
    • Tektin-B1 antibody
    • Tektin-t antibody
    • Testicular tektin antibody
    • Testicular tektin B1 like protein antibody
    • Testicular tektin B1-like protein antibody
    see all

Anti-TEKT2 antibody images

  • Anti-TEKT2 antibody (ab60918) + immunogen

    Predicted band size : 50 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab60918 staining human Hela cells by ICC/IF. Cells were methanol or formaldehyde fixed and not permeabilized prior to blocking in 2% BSA for 30 minutes. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour. The secondary antibody was Alexa Fluor® 594 conjugated donkey polyclonal to mouse, diluted 1/1000.

    See Abreview

References for Anti-TEKT2 antibody (ab60918)

ab60918 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2%
Username

Abcam user community

Verified customer

Submitted Nov 27 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"