The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionStructural component of ciliary and flagellar microtubules. Plays a key role in the assembly or attachment of the inner dynein arm to microtubules in sperm flagella and tracheal cilia. Forms filamentous polymers in the walls of ciliary and flagellar microtubules.
Tissue specificityExpressed at high levels in testis, trachea and fetal lung, and at lower levels in ovary, pituitary, adult lung, fetal brain and fetal kidney.
Sequence similaritiesBelongs to the tektin family.
Cellular localizationCell projection > cilium > flagellum. Cytoplasm > cytoskeleton > cilium axoneme. Cytoplasm > cytoskeleton > flagellum axoneme. In sperm, observed in a discontinuous punctate pattern in the flagellum and in the postacrosomal head region.