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Recombinant full length protein (Human) from insect cells.
Our Abpromise guarantee covers the use of ab5181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1-2µg for 106 cells. (Also see Abreview)|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. Fix with 1:1 ice cold MEOH:acetone and place at -20°C for 10 minutes.|
|WB||1/500 - 1/1000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
An additional background band at ~110 kDa may be present. Please note the isotype for this product is IgM and requires the use of an IgM secondary. Even though we have a positive Abreview on IHC, this is not a batch tested application and we cannot guarantee that it will work.
|IP||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"