Anti-Telomerase reverse transcriptase antibody [2C4] (ab5181)
- Product nameAnti-Telomerase reverse transcriptase antibody [2C4]See all Telomerase reverse transcriptase primary antibodies ...
- DescriptionMouse monoclonal [2C4] to Telomerase reverse transcriptase
- Tested applicationsFlow Cyt, IHC-P, ICC/IF, WB, IP more details
- Species reactivityReacts with: Human
Recombinant full length protein (Human) from insect cells.
- Positive control
- Immunofluorescence/immunocytochemistry: 293T, Hela, BJ, WI-38 Western Blot: 293T Whole Cell Lysates, Hela Whole Cell Lysates Immunohistochemistry-Paraffin: Pancreatic carcinoma, Normal pancreas (human)
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, Raw Ascites
- Concentration information loading...
- PurityConcentrated Culture Supernatant
- Clonality Monoclonal
- Clone number2C4
- Research Areas
Our Abpromise guarantee covers the use of ab5181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Flow Cyt: Use 1-2µg for 106 cells. (Also see Abreview)|
|IHC-P||IHC-P: Use at an assay dependent concentration.|
|ICC/IF||ICC/IF: Use at an assay dependent concentration. Fix with 1:1 ice cold MEOH:acetone and place at -20°C for 10 minutes.|
|WB||WB: 1/500 - 1/1000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
An additional background band at ~110 kDa may be present. Even though we have a positive Abreview on IHC, this is not a batch tested application and we cannot guarantee that it will work.
|IP||IP: Use at an assay dependent concentration.|
- FunctionTelomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
- Tissue specificityExpressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T lymphocytes, and at a low to undetectable level in peripheral blood T lymphocytes.
- Involvement in diseaseNote=Activation of telomerase has been implicated in cell immortalization and cancer cell pathogenesis.
Defects in TERT are associated with susceptibilty to aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Note=Genetic variations in TERT are associated with coronary artery disease (CAD).
Defects in TERT are a cause of dyskeratosis congenita autosomal dominant (ADDKC) [MIM:127550]; also known as dyskeratosis congenita Scoggins type. ADDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
Defects in TERT are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF) [MIM:178500]. Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease.
- Sequence similaritiesBelongs to the reverse transcriptase family. Telomerase subfamily.
Contains 1 reverse transcriptase domain.
- DomainThe primer grip sequence in the RT domain is required for telomerase activity and for stable association with short telomeric primers.
The RNA-interacting domain 1 (RD1)/N-terminal extension (NTE) is required for interaction with the pseudoknot-template domain of each of TERC dimers. It contains anchor sites that bind primer nucleotides upstream of the RNA-DNA hybrid and is thus an essential determinant of repeat addition processivity.
The RNA-interacting domain 2 (RD2) is essential for both interaction with the CR4-CR5 domain of TERC and for DNA sythesis.
modificationsUbiquitinated, leading to proteasomal degradation.
Phosphorylation at Tyr-707 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation by the AKT pathway promotes nuclear location.
- Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Chromosome > telomere. Cytoplasm. Nucleus > PML body. Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress-mediated phosphorylation at Tyr-707. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT.
- EST2 antibody
- hEST2 antibody
- htert antibody
- TCS1 antibody
- Telomerase associated protein 2 antibody
- Telomerase Catalytic Subunit antibody
- Telomerase reverse transcriptase antibody
- Telomerase-associated protein 2 antibody
- Telomere Reverse Transcriptase antibody
- TERT antibody
- TERT_HUMAN antibody
- TP2 antibody
- TRT antibody
Anti-Telomerase reverse transcriptase antibody [2C4] images
Predicted band size : 127 kDa
ab5181 at a 1/500 dilution staining approximately 127kDa Telomerase in MJ90 cell lysate by Western blot (ECL).
Overlay histogram showing Jurkat cells stained with ab5181 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5181, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-Telomerase reverse transcriptase antibody [2C4] (ab5181)
This product has been referenced in:
- Hemmaid KZ et al. Impact of Hepatitis C Virus (HCV) infection on biomolecular markers influencing the pathogenesis of bladder cancer. Infect Agent Cancer 8:24 (2013). IHC ; Human . Read more (PubMed: 23809295) »
- Cheung DH et al. PinX1 is involved in telomerase recruitment and regulates telomerase function by mediating its localization. FEBS Lett 586:3166-71 (2012). Read more (PubMed: 22749911) »