Recombinant
RabMAb

Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)

Overview

  • Product name
    Anti-Telomerase reverse transcriptase antibody [Y182]
    See all Telomerase reverse transcriptase primary antibodies
  • Description
    Rabbit monoclonal [Y182] to Telomerase reverse transcriptase
  • Tested applications
    Suitable for: WB, Flow Cyt, IP, IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C terminus of human Telomerase reverse transcriptase.

  • Positive control
    • WB: Hela cell lysate. IHC-P: Human lung adenocarcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab32020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 127 kDa).
Flow Cyt 1/100.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 20367640
IHC-Fr Use a concentration of 0.75 µg/ml. PubMed: 17982423
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
    • Tissue specificity
      Expressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T lymphocytes, and at a low to undetectable level in peripheral blood T lymphocytes.
    • Involvement in disease
      Note=Activation of telomerase has been implicated in cell immortalization and cancer cell pathogenesis.
      Defects in TERT are associated with susceptibilty to aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
      Note=Genetic variations in TERT are associated with coronary artery disease (CAD).
      Defects in TERT are a cause of dyskeratosis congenita autosomal dominant (ADDKC) [MIM:127550]; also known as dyskeratosis congenita Scoggins type. ADDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
      Defects in TERT are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF) [MIM:178500]. Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease.
    • Sequence similarities
      Belongs to the reverse transcriptase family. Telomerase subfamily.
      Contains 1 reverse transcriptase domain.
    • Domain
      The primer grip sequence in the RT domain is required for telomerase activity and for stable association with short telomeric primers.
      The RNA-interacting domain 1 (RD1)/N-terminal extension (NTE) is required for interaction with the pseudoknot-template domain of each of TERC dimers. It contains anchor sites that bind primer nucleotides upstream of the RNA-DNA hybrid and is thus an essential determinant of repeat addition processivity.
      The RNA-interacting domain 2 (RD2) is essential for both interaction with the CR4-CR5 domain of TERC and for DNA sythesis.
    • Post-translational
      modifications
      Ubiquitinated, leading to proteasomal degradation.
      Phosphorylation at Tyr-707 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation by the AKT pathway promotes nuclear location.
    • Cellular localization
      Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Chromosome > telomere. Cytoplasm. Nucleus > PML body. Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress-mediated phosphorylation at Tyr-707. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMM9 antibody
      • DKCA2 antibody
      • DKCB4 antibody
      • EST2 antibody
      • HEST2 antibody
      • htert antibody
      • hTRT antibody
      • PFBMFT1 antibody
      • TCS1 antibody
      • Telomerase associated protein 2 antibody
      • Telomerase catalytic subunit antibody
      • Telomerase reverse transcriptase antibody
      • Telomerase-associated protein 2 antibody
      • Telomere Reverse Transcriptase antibody
      • TERT antibody
      • TERT_HUMAN antibody
      • TP2 antibody
      • TRT antibody
      see all

    Images

    • ab32020 staining Telomerase reverse transcriptase in human thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% hydrogen peroxide for 10 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer pH6.0 at 100°C for 20 minutes. Samples were incubated with primary antibody (1/100) for 20 minutes at 25°C. An undiluted HRP polymer-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020) at 1/1000 dilution + Hela cell lysate

      Predicted band size : 127 kDa
      Observed band size : 122 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Im E  et al. Human Telomerase Reverse Transcriptase (hTERT) Positively Regulates 26S Proteasome Activity. J Cell Physiol N/A:N/A (2016). Read more (PubMed: 27648923) »
    • Yang Y  et al. MR molecular imaging of tumours using ferritin heavy chain reporter gene expression mediated by the hTERT promoter. Eur Radiol 26:4089-4097 (2016). Read more (PubMed: 26960542) »

    See all 40 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH6.0, 100C, 20 min
    Sample
    Human Tissue sections (thymus)
    Specification
    thymus
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 30 2014

    Application
    Western blot
    Loading amount
    25000 cells
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (8)
    Sample
    Human Cell lysate - whole cell (prostate)
    Specification
    prostate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 30 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Sample
    Marmoset (common) Tissue sections (Adult testis)
    Specification
    Adult testis
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    煜欽 林

    Verified customer

    Submitted Jun 21 2013

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (corneal endothelium)
    Permeabilization
    No
    Specification
    corneal endothelium
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
    Fixative
    Formaldehyde
    Username

    Dr. Zhiguo He

    Verified customer

    Submitted Nov 01 2012

    Application
    Immunocytochemistry
    Sample
    Cow Cultured Cells (Corneal progenitor cells)
    Specification
    Corneal progenitor cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton X
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 01 2012

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Breast carcinoma)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    Breast carcinoma
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 23 2009

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (adipose stroma)
    Permeabilization
    Yes - 0.15% Triton-X/PBS
    Specification
    adipose stroma
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 05 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (293T)
    Total protein in input
    250 µg
    Specification
    293T
    Immuno-precipitation step
    Protein A
    Username

    Abcam user community

    Verified customer

    Submitted Dec 03 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS and 293T cell line)
    Loading amount
    30 µg
    Specification
    U2OS and 293T cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 02 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up