Overview

  • Product nameAnti-TEM1 antibody
    See all TEM1 primary antibodies
  • Description
    Rabbit polyclonal to TEM1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TEM1.

    (Peptide available as ab48184.)

  • Positive control
    • This antibody gave a positive signal in the following Mouse Tissue Lysates: Skeletal Muscle and Heart

Properties

Applications

Our Abpromise guarantee covers the use of ab48185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 80 kDa).

Target

  • FunctionMay play a role in tumor angiogenesis.
  • Tissue specificityExpressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts.
  • Sequence similaritiesContains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 1 Sushi (CCP/SCR) domain.
  • Post-translational
    modifications
    O-glycosylated with sialylated oligosaccharides.
    May be N-glycosylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD164 sialomucin like 1 antibody
    • CD164L1 antibody
    • CD248 antibody
    • CD248 antigen endosialin antibody
    • CD248 molecule endosialin antibody
    • CD248_HUMAN antibody
    • Endosialin antibody
    • TEM 1 antibody
    • TEM1 antibody
    • Tumor endothelial marker 1 antibody
    see all

Anti-TEM1 antibody images

  • All lanes : Anti-TEM1 antibody (ab48185) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Mouse)
    Lane 2 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 92 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 250 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TEM1 antibody (ab48185)

ab48185 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"