Anti-TEM1 antibody (ab67273)


  • Product nameAnti-TEM1 antibody
    See all TEM1 primary antibodies
  • Description
    Rabbit polyclonal to TEM1
  • Specificityab67273 recognizes both isoforms of TEM1.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 14 amino acid synthetic peptide derived from near the carboxy terminus of the human TEM1.

  • Positive control
    • Human colon tissue lysate and human colon tissue.



Our Abpromise guarantee covers the use of ab67273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-P: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 & 50 kDa (predicted molecular weight: 80 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play a role in tumor angiogenesis.
    • Tissue specificityExpressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts.
    • Sequence similaritiesContains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 1 Sushi (CCP/SCR) domain.
    • Post-translational
      O-glycosylated with sialylated oligosaccharides.
      May be N-glycosylated.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD164 sialomucin like 1 antibody
      • CD164L1 antibody
      • CD248 antibody
      • CD248 antigen endosialin antibody
      • CD248 molecule endosialin antibody
      • CD248_HUMAN antibody
      • Endosialin antibody
      • TEM 1 antibody
      • TEM1 antibody
      • Tumor endothelial marker 1 antibody
      see all

    Anti-TEM1 antibody images

    • Lane 1 : Anti-TEM1 antibody (ab67273) at 0.5 µg/ml
      Lane 2 : Anti-TEM1 antibody (ab67273) at 1 µg/ml

      Lane 1 : Human colon tissue lysate
      Lane 2 : Human colon tissue lysate

      Lysates/proteins at 15 µg per lane.

      anti rabbit secondary antibody

      Predicted band size : 50, 80 kDa
      Observed band size : 50,80 kDa
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
    • ab67273 at 2.5 μg/ staining of TEM1 in human colon tissue using IHC-P.
    • Immunofluorescence of TEM1 in Human Colon cells using ab67273 at 20 ug/ml.

    References for Anti-TEM1 antibody (ab67273)

    ab67273 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us yesterday and alerting us to the problem you are experiencing with the anti-TEM1 antibody (ab67273). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (skin)
    Specification skin
    Fixative Paraformaldehyde
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: proteinase K
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Jan 09 2012

    Thank you for contacting us.

    Lab has confirmed that they have tested this antibody in western blotting and Immunohistochemistry only - no further tests other than these has been done. I am sorry the data we have is already published on the dat...

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