Anti-TEM1 antibody (ab67275)

Overview

  • Product nameAnti-TEM1 antibody
    See all TEM1 primary antibodies
  • Description
    Rabbit polyclonal to TEM1
  • Specificityab67275 recognizes only the larger isoform of TEM1.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 14 amino acid synthetic peptide from near the amino terminus of the human TEM1.

  • Positive control
    • Human colon tissue lysate. Human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab67275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).

Target

  • FunctionMay play a role in tumor angiogenesis.
  • Tissue specificityExpressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts.
  • Sequence similaritiesContains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 1 Sushi (CCP/SCR) domain.
  • Post-translational
    modifications
    O-glycosylated with sialylated oligosaccharides.
    May be N-glycosylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD164 sialomucin like 1 antibody
    • CD164L1 antibody
    • CD248 antibody
    • CD248 antigen endosialin antibody
    • CD248 molecule endosialin antibody
    • CD248_HUMAN antibody
    • Endosialin antibody
    • TEM 1 antibody
    • TEM1 antibody
    • Tumor endothelial marker 1 antibody
    see all

Anti-TEM1 antibody images

  • Lane 1 : Anti-TEM1 antibody (ab67275) at 0.5 µg/ml
    Lane 2 : Anti-TEM1 antibody (ab67275) at 1 µg/ml

    Lane 1 : Human colon tissue lysate
    Lane 2 : Human colon tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Anti rabbit secondary antibody

    Predicted band size : 80 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  •  ab67275 at 2.5 μg/ml of TEM1 in human colon tissue using IHC-P.
  • Immunofluorescence of TEM1 in Human Colon cells using ab67275 at 20 ug/ml.

References for Anti-TEM1 antibody (ab67275)

ab67275 has not yet been referenced specifically in any publications.

Product Wall

Human colon tissue was one of the tissues tested for the original WB screen. When it turned out to be a positive control, it was also tested in IHC. Human colon tissue also turned out to be a positive control for IHC. The laboratory technician confir...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Spleen and embryonic fibroblast)
Specification Spleen and embryonic fibroblast
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. Sian Lax

Verified customer

Submitted May 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"