Anti-TEM7 antibody [197C193] (ab13667)

Overview

  • Product name
    Anti-TEM7 antibody [197C193]
    See all TEM7 primary antibodies
  • Description
    Mouse monoclonal [197C193] to TEM7
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:

    NNLSPKTKGTPVHLGTI

    , corresponding to amino acids 411-427 of Human TEM7.

  • Positive control
    • HCT-116 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notes
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
    Used at a dilution of 1/1500 for 16 hrs on human brain tumor (see Abreview for further information).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Plays a critical role in endothelial cell capillary morphogenesis.
    • Tissue specificity
      Detected in endothelial cells from colorectal cancer, and in endothelial cells from primary cancers of the lung, liver, pancreas, breast and brain. Not detectable in endothelial cells from normal tissue. Expressed in fibrovascular membrane with increased expression in individuals with proliferative diabetic retinopathy.
    • Sequence similarities
      Belongs to the plexin family.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localization
      Cytoplasm; Secreted and Cell membrane. Cell junction > tight junction. Localized predominantly at the tight junctions of vascular endothelial cells and to a lesser extent at the luminal surface of vascular endothelial cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686F0937 antibody
      • FLJ36270 antibody
      • FLJ45632 antibody
      • OTTHUMP00000164240 antibody
      • Plexin domain containing 1 antibody
      • Plexin domain containing protein 1 precursor antibody
      • Plexin domain-containing protein 1 antibody
      • Plxdc1 antibody
      • PXDC1_HUMAN antibody
      • TEM3 antibody
      • TEM7 antibody
      • Tumor endothelial marker 3 antibody
      • Tumor endothelial marker 7 antibody
      see all

    Anti-TEM7 antibody [197C193] images

    • ab13667 at a 1/1500 dilution staining human brain tumor (glioblatoma) samples by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Heat mediated antigen retrieval was used and the tissue was fixed with ethanol and blocked with 2.5% serum prior to incubation with the antibody for 16 hours. A pan-specifc universal secondary antibody was used.

      See Abreview

    References for Anti-TEM7 antibody [197C193] (ab13667)

    ab13667 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain tumor)
    Specification
    Brain tumor
    Fixative
    Acetone
    Antigen retrieval step
    Heat mediated
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2.5
    Username

    Abcam user community

    Verified customer

    Submitted Jul 13 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up