The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesSandwich ELISA and ELISA: Use at a concentration of 10 µg/ml.
WB: 1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays a critical role in endothelial cell capillary morphogenesis.
Tissue specificityDetected in endothelial cells from colorectal cancer, and in endothelial cells from primary cancers of the lung, liver, pancreas, breast and brain. Not detectable in endothelial cells from normal tissue. Expressed in fibrovascular membrane with increased expression in individuals with proliferative diabetic retinopathy.
Sequence similaritiesBelongs to the plexin family.
Cellular localizationCytoplasm; Secreted and Cell membrane. Cell junction > tight junction. Localized predominantly at the tight junctions of vascular endothelial cells and to a lesser extent at the luminal surface of vascular endothelial cells.