Overview

  • Product name
    Anti-TEM8 antibody [200C1339]
    See all TEM8 primary antibodies
  • Description
    Mouse monoclonal [200C1339] to TEM8
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    CINFTRVKNNQPAKYPL

    , corresponding to amino acids 481-497 of Human TEM8.

  • Positive control
    • Tested with TEM8 transfected cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 84 kDa.

A 70 kD band was seen when cell extracts were treated with a glycosidase cocktail. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

IHC-P 1/200 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.
  • Tissue specificity
    Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells.
  • Involvement in disease
    Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similarities
    Belongs to the ATR family.
    Contains 1 VWFA domain.
  • Domain
    Binding to PA occurs through the VWA domain.
  • Cellular localization
    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anthrax toxin receptor 1 antibody
    • ANTR1_HUMAN antibody
    • Antxr1 antibody
    • ATR antibody
    • Tumor endothelial marker 8 antibody
    see all

Anti-TEM8 antibody [200C1339] images

  • Western blot detection of TEM8 in TEM8 transfected cell lysate using ab13798 at 1 µg/ml.
  • Formalin-fixed, paraffin-embedded human colon stained with ab13798 diluted 1:200, detection using peroxidase-conjugated secondary antibody and DAB chromogen.
  • Formalin-fixed, paraffin-embedded human colon stained with ab13798 diluted 1:200, detection using peroxidase-conjugated secondary and DAB chromogen.

References for Anti-TEM8 antibody [200C1339] (ab13798)

This product has been referenced in:
  • Sakai Y  et al. Bcl-2 mediated modulation of vascularization in prostate cancer xenografts. Prostate 69:459-70 (2009). WB ; Human . Read more (PubMed: 19107861) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Dog Cell lysate - whole cell (Hemangiosarcoma cell lines)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
Hemangiosarcoma cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Holly Pondenis

Verified customer

Submitted Feb 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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