Overview

  • Product nameAnti-TEM8 antibody
    See all TEM8 primary antibodies
  • Description
    Rabbit polyclonal to TEM8
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    LMKLTEDRE QIRQGLE

    , corresponding to amino acids 92-107 of Human TEM8.

  • Positive control
    • WB: mouse kidney lysate IHC: mouse lung, skin and intestine IF/ICC: HepG2 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab19387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 63 kDa).

We recommend using 5% BSA as your blocking buffer and antibody diluent.

Target

  • FunctionPlays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.
  • Tissue specificityDetected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells.
  • Involvement in diseaseDefects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similaritiesBelongs to the ATR family.
    Contains 1 VWFA domain.
  • DomainBinding to PA occurs through the VWA domain.
  • Cellular localizationCell membrane. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anthrax toxin receptor 1 antibody
    • ANTR1_HUMAN antibody
    • Antxr1 antibody
    • ATR antibody
    • Tumor endothelial marker 8 antibody
    see all

Anti-TEM8 antibody images

  • ICC/IF image of ab19387 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19387, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TEM8 antibody (ab19387)

This product has been referenced in:

See all 4 Publications for this product

Product Wall

Thank you for contacting us. The TEM8 antibody ab19387 was raised against a specific region in the mouse TEM8 that does not show homology to CMG2 (also known as ATR2). I hope this information is helpful to you. Please do not hesitate to contact ...

Read More

Thank you for your reply and providing more information and a whole blot image. Your problem puzzled me greatly and I also asked other colleagues to help understand what might be happening. Here are a few of our thoughts: -For the western b...

Read More

I'm sorry to hear you are experiencing unsatisfactory results with ab19387. I would be happy to consider offering you a replacement vial of ab21270, however before I can do that I would like to investigate this matter further to make sure that the ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"