Anti-TEM8/ATR antibody (ab21269)
Key features and details
- Rabbit polyclonal to TEM8/ATR
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TEM8/ATR antibody
See all TEM8/ATR primary antibodies -
Description
Rabbit polyclonal to TEM8/ATR -
Host species
Rabbit -
Specificity
This antibody will recognize only the largest isoform of TEM8/ATR.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TEM8/ATR (C terminal).
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Positive control
- WB: K562 cell lysates, Wild-type HeLa cell lysate, U-2 OS cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
This antibody is ion exchange chromatography purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 2 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 63 kDa.
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Notes |
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ICC/IF
Use a concentration of 2 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 63 kDa. |
Target
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Function
Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. -
Tissue specificity
Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells. -
Involvement in disease
Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
Sequence similarities
Belongs to the ATR family.
Contains 1 VWFA domain. -
Domain
Binding to PA occurs through the VWA domain. -
Cellular localization
Cell membrane. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia. - Information by UniProt
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Database links
- Entrez Gene: 84168 Human
- Omim: 606410 Human
- SwissProt: Q9H6X2 Human
- Unigene: 165859 Human
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Alternative names
- Anthrax toxin receptor 1 antibody
- ANTR1_HUMAN antibody
- Antxr1 antibody
see all
Images
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All lanes : Anti-TEM8/ATR antibody (ab21269) at 1 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ANTXR1 knockout HeLa cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : U-2 OS cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-TEM8/ATR antibody staining at 1 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab21269 was shown to bind specifically to TEM8/ATR. A band was observed at 100 kDa in wild-type HeLa cell lysates with no signal observed at this size in ANTXR1 knockout cell line ab265077 (knockout cell lysate ab257350). To generate this image, wild-type and ANTXR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Lane 1 : Anti-TEM8/ATR antibody (ab21269) at 0.5 µg/ml
Lane 2 : Anti-TEM8/ATR antibody (ab21269) at 1 µg/ml
Lane 3 : Anti-TEM8/ATR antibody (ab21269) at 2 µg/ml
All lanes : K562 cell lysate
Predicted band size: 63 kDaWestern blot analysis of TEM8/ATR in K562 cell lysates with ab21269 at (A) 0.5, (B) 1 and (C) 2µg/ml.
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ab21269 at 2µg/ml staining TEM8/ATR in K562 cells by ICC/IF
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab21269 has been referenced in 2 publications.
- Zhang Q et al. Improved Antitumor Efficacy of Combined Vaccine Based on the Induced HUVECs and DC-CT26 Against Colorectal Carcinoma. Cells 8:N/A (2019). PubMed: 31121964
- Hu K et al. Cell autonomous ANTXR1-mediated regulation of extracellular matrix components in primary fibroblasts. Matrix Biol 62:105-114 (2017). PubMed: 28011198