Overview

  • Product name
    TEMED
  • Description
    Free radical stabilizer used as a catalyst with APS to promote polymerization of acrylamide gels.
  • Alternative names
    • 1,2-Bis(dimethylamino)ethane
    • N,N,N',N'-tetramethylethane-1,2-diamine
    • Tetramethylethylenediamine
    • TMEDA
  • Biological description

    Tertiary amine base used to catalyze the formation of free radicals from ammonium persulfate (APS) or riboflavin. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules such as nucleic acids and proteins.


     

  • Purity
    > 99%

Properties

  • Chemical name
    N,N,N',N'-Tetramethyl-1,2-ethanediamine
  • Molecular weight
    116.21
  • Chemical structure
    Chemical Structure
  • Molecular formula
    C6H16N2
  • CAS Number
    110-18-9
  • Storage instructions
    Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:
  • Faulkner RD  et al. Preparative electrophoresis of histones in solubilizable polyacrylamide gels. Biochim Biophys Acta 708:245-52 (1982). Read more (PubMed: 7171616) »
  • Allison JH  et al. Effect of N,N,N',N'-tetramethylethylenediamine on the migration of proteins in SDS polyacrylamide gels. Anal Biochem 58:592-601 (1974). Read more (PubMed: 4827399) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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