Overview

  • Product nameAnti-Tenascin C antibody [BC-24]
    See all Tenascin C primary antibodies
  • Description
    Mouse monoclonal [BC-24] to Tenascin C
  • SpecificityBC-24 reacts specifically with tenascin using ELISA, RIA and immunoblotting procedures. The product reacts with tenascin from frozen sections as well as protease-digested formalin-fixed, paraffin-embedded, Bouin's-, Brunnel's-, B5-, alcohol-acetic acid- and Susa solution-fixed tissues. The antibody recognizes all isoforms of human tenascin.
  • Tested applicationsSuitable for: WB, ELISA, IHC-FoFr, IHC-P, RIA, IHC-Fr, ICC, Dot blot, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

  • EpitopeBC-24 recognizes an epitope located within the N-terminal EGF-like sequence of the human tenascin molecule.
  • Positive control
    • Tenascin is synthesized by fibroblasts, chondroblasts, osteocytes, smooth muscle cells and glial cells.
  • General notes


    Tenascin (TN)(Erickson and Bourdon 1989, Erickson 1993) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1-200/220, myotendinous antigen (MI), neuronectin (NEC1) and glioma mesenchymal extracellular matrix (GMEM). The TN molecule is a disulfide-linked hexamer. Human TN has 3 subunits of 190, 200 and 220 kDa. It is primarily made up of 14.5 epidermal growth-factor-like repeats, 15 units similar to the fibronectin type-III-homology repeat and, at the C-terminus, has a sequence with homology to the globular domain of the beta and gamma chain of fibrinogen. Monoclonal antibody reacting specifically with tenascin is an essential tool for the localization, identification and studies on the role of the molecule in epithelial-mesenchymal and neuronal-glial interactions (Castellucci et al. 1991, Balza et al. 1993).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: Ascites
  • Concentration information loading...
  • PurityAscites
  • Primary antibody notesTenascin (TN)(Erickson and Bourdon 1989, Erickson 1993) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1-200/220, myotendinous antigen (MI), neuronectin (NEC1) and glioma mesenchymal extracellular matrix (GMEM). The TN molecule is a disulfide-linked hexamer. Human TN has 3 subunits of 190, 200 and 220 kDa. It is primarily made up of 14.5 epidermal growth-factor-like repeats, 15 units similar to the fibronectin type-III-homology repeat and, at the C-terminus, has a sequence with homology to the globular domain of the beta and gamma chain of fibrinogen. Monoclonal antibody reacting specifically with tenascin is an essential tool for the localization, identification and studies on the role of the molecule in epithelial-mesenchymal and neuronal-glial interactions (Castellucci et al. 1991, Balza et al. 1993).
  • ClonalityMonoclonal
  • Clone numberBC-24
  • Myelomaunknown
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200.
ELISA Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/4000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
RIA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 24386267

Target

  • FunctionExtracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
  • Sequence similaritiesBelongs to the tenascin family.
    Contains 15 EGF-like domains.
    Contains 1 fibrinogen C-terminal domain.
    Contains 15 fibronectin type-III domains.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 150 225 antibody
    • Cytotactin antibody
    • Glioma associated extracellular matrix antigen antibody
    • Glioma-associated-extracellular matrix antigen antibody
    • GMEM antibody
    • GP 150 225 antibody
    • GP 150-225 antibody
    • GP antibody
    • Hexabrachion antibody
    • HXB antibody
    • JI antibody
    • MGC167029 antibody
    • Miotendinous antigen antibody
    • Myotendinous antigen antibody
    • Neuronectin antibody
    • TENA_HUMAN antibody
    • Tenascin antibody
    • Tenascin-C antibody
    • Tenascin-C isoform 14/AD1/16 antibody
    • TenascinC antibody
    • TN antibody
    • TN C antibody
    • TN-C antibody
    • TNC antibody
    see all

References for Anti-Tenascin C antibody [BC-24] (ab6393)

This product has been referenced in:
  • Rehmann MS  et al. Tuning microenvironment modulus and biochemical composition promotes human mesenchymal stem cell tenogenic differentiation. J Biomed Mater Res A N/A:N/A (2016). Read more (PubMed: 26748903) »
  • Su K  et al. Induction of endometrial mesenchymal stem cells into tissue-forming cells suitable for fascial repair. Acta Biomater 10:5012-20 (2014). Read more (PubMed: 25194931) »

See all 13 Publications for this product

Product Wall

Thank you for your patience. I have contacted the originators of ab3970, ab6393, and ab7839 and have received the following replies: ab3970 - This antibody reacts with tenascin; it was raised against mammary tumor of human origin. Tenascin-C cou...

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Thank you for your recent phone call and your interest in our tenascin and myosin heavy chain antibodies. As we discussed we do not currently have antibodies which have been tested in chicken tissue and the homology searches revealed that for tenas...

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Thank you for your reply and providing more details. As I understand, you have used purified human Tenascin C (positive control) and the antibody recognized the target; however it failed to work in the samples. Do the pancreatic tumour cells expres...

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Thank you for getting back to us and providing more information about your assay. We have read through your protocol very carefully and would like to ask two more questions which could help us to identify the source of the problem. 1. Has the p...

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This antibody has only been tested against the human antigen. Recognition of rat tenascin is unknown.

This antibody has only been tested against the human antigen. Recognition of rat tenascin is unknown.

If tenascin is cell surface then there is no reason that FACS will not work. Some FACS applications use a secondary conjugated antibody, so this should work also.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"