Overview

  • Product nameAnti-Tenascin C antibody [DB7]
    See all Tenascin C primary antibodies
  • Description
    Mouse monoclonal [DB7] to Tenascin C
  • Specificityab86182 reacts with the fibrinogen like knob domain of Tenascin C.
  • Tested applicationsSuitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tenascin polypeptides from spent culture supernatant of human fibroblasts isolated by affinity chromatography.

  • General notes


    ab86182 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab86182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 241 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionExtracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
  • Sequence similaritiesBelongs to the tenascin family.
    Contains 15 EGF-like domains.
    Contains 1 fibrinogen C-terminal domain.
    Contains 15 fibronectin type-III domains.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 150 225 antibody
    • Cytotactin antibody
    • Glioma associated extracellular matrix antigen antibody
    • Glioma-associated-extracellular matrix antigen antibody
    • GMEM antibody
    • GP 150 225 antibody
    • GP 150-225 antibody
    • GP antibody
    • Hexabrachion antibody
    • HXB antibody
    • JI antibody
    • MGC167029 antibody
    • Miotendinous antigen antibody
    • Myotendinous antigen antibody
    • Neuronectin antibody
    • TENA_HUMAN antibody
    • Tenascin antibody
    • Tenascin-C antibody
    • Tenascin-C isoform 14/AD1/16 antibody
    • TenascinC antibody
    • TN antibody
    • TN C antibody
    • TN-C antibody
    • TNC antibody
    see all

Anti-Tenascin C antibody [DB7] images

  • Immunohistochemical analysis of Human cornea tissue, staining Tenascin C wih ab86182.

    Tissue was taken from healthy patients (left) or patients with granular dystrophy (right). Sections were blocked with blocking solution for 30 minutes at room temperature before incubation with primary antibody (1/50) overnight at 4°C. Staining was detected with DAB.

References for Anti-Tenascin C antibody [DB7] (ab86182)

This product has been referenced in:
  • Szalai E  et al. Fibrillin-2, tenascin-C, matrilin-2, and matrilin-4 are strongly expressed in the epithelium of human granular and lattice type I corneal dystrophies. Mol Vis 18:1927-36 (2012). IHC-P ; Human . Read more (PubMed: 22876117) »
  • Tiitta O  et al. Enhanced tenascin expression in cervical and vulvar koilocytotic lesions. Am J Pathol 141:907-13 (1992). Read more (PubMed: 1384340) »

See all 2 Publications for this product

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Es importante que todos los anticuerpos que escojas para llevar a cabo IHC-P en tejido humano estén validados (y por tanto garantizados por Abcam) para funcionar en esta especie y aplicación. Est...

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