Overview

  • Product name
    Anti-Tenascin C antibody [EB2]
    See all Tenascin C primary antibodies
  • Description
    Mouse monoclonal [EB2] to Tenascin C
  • Specificity
    ab88280 reacts with the 4th and 5th fibronectin repeats of human Tenascin C polypeptides.
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Rabbit, Guinea pig, Human
  • Immunogen

    Purified Tenascin C from human fetal fibroblasts.

  • Positive control
    • Vulva carcinoma
  • General notes


    ab88280 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 241 kDa.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000.

Target

  • Function
    Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
  • Sequence similarities
    Belongs to the tenascin family.
    Contains 15 EGF-like domains.
    Contains 1 fibrinogen C-terminal domain.
    Contains 15 fibronectin type-III domains.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 150 225 antibody
    • Cytotactin antibody
    • Glioma associated extracellular matrix antigen antibody
    • Glioma-associated-extracellular matrix antigen antibody
    • GMEM antibody
    • GP 150 225 antibody
    • GP 150-225 antibody
    • GP antibody
    • Hexabrachion antibody
    • HXB antibody
    • JI antibody
    • MGC167029 antibody
    • Miotendinous antigen antibody
    • Myotendinous antigen antibody
    • Neuronectin antibody
    • TENA_HUMAN antibody
    • Tenascin antibody
    • Tenascin-C antibody
    • Tenascin-C isoform 14/AD1/16 antibody
    • TenascinC antibody
    • TN antibody
    • TN C antibody
    • TN-C antibody
    • TNC antibody
    see all

Anti-Tenascin C antibody [EB2] images

  • ab88280, staining Tenascin C in vulva carcinoma frozen tissue sections by Immunohistochemistry.

References for Anti-Tenascin C antibody [EB2] (ab88280)

This product has been referenced in:
  • Canseco JA  et al. Effect on ligament marker expression by direct-contact co-culture of mesenchymal stem cells and anterior cruciate ligament cells. Tissue Eng Part A 18:2549-58 (2012). Read more (PubMed: 22780864) »
  • Angunawela RI  et al. Refractive lenticule re-implantation after myopic ReLEx: a feasibility study of stromal restoration after refractive surgery in a rabbit model. Invest Ophthalmol Vis Sci 53:4975-85 (2012). Read more (PubMed: 22743323) »

See all 2 Publications for this product

Product Wall

Thank you for submitting an Abreview of ab88280. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a untested application, we would like to follow up on this to ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast Cancer Cell Lines)
Specification
Breast Cancer Cell Lines
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: PH9/6
Permeabilization
No
Username

Mr. Stefan Swierczynski

Verified customer

Submitted Jun 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast Cancer Cell Line MDA-MB231)
Specification
Breast Cancer Cell Line MDA-MB231
Fixative
Methanol
Permeabilization
No
Username

Mr. Stefan Swierczynski

Verified customer

Submitted Jun 07 2012

DISCOUNT CODE: 000000

Ablaufdatum: 21. September 2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab88280 inIHC-P zu testen, annehmen möchten. Wie am gewünscht, können Sie den Gutschein...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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