Recombinant
RabMAb

Anti-Tenascin C antibody [EPR4219] (ab108930)

Overview

  • Product name
    Anti-Tenascin C antibody [EPR4219]
    See all Tenascin C primary antibodies
  • Description
    Rabbit monoclonal [EPR4219] to Tenascin C
  • Specificity
    IHC on human tissues which we tested (such as testis, pancreas and stomach) showed non-specific staining. We don’t recommend this antibody for IHC on human tissues.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Tenascin C.

  • Positive control
    • Human fetal brain lysate, U87-MG (human glioblastoma) whole cell lysate, mouse cerebellum, mouse cerebellar cortex and rat cerebellar cortex.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 241 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 24384131

Antigen retrieval is recommended.

IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
    • Sequence similarities
      Belongs to the tenascin family.
      Contains 15 EGF-like domains.
      Contains 1 fibrinogen C-terminal domain.
      Contains 15 fibronectin type-III domains.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • 150 225 antibody
      • Cytotactin antibody
      • Glioma associated extracellular matrix antigen antibody
      • Glioma-associated-extracellular matrix antigen antibody
      • GMEM antibody
      • GP 150 225 antibody
      • GP 150-225 antibody
      • GP antibody
      • Hexabrachion antibody
      • HXB antibody
      • JI antibody
      • MGC167029 antibody
      • Miotendinous antigen antibody
      • Myotendinous antigen antibody
      • Neuronectin antibody
      • TENA_HUMAN antibody
      • Tenascin antibody
      • Tenascin-C antibody
      • Tenascin-C isoform 14/AD1/16 antibody
      • TenascinC antibody
      • TN antibody
      • TN C antibody
      • TN-C antibody
      • TNC antibody
      see all

    Anti-Tenascin C antibody [EPR4219] images

    • Immunohistochemistry (Frozen sections) analysis of Mouse E14 spinal cord labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilised with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counter stain. Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA Buffer, PH9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counter stain. Staining on the molecular layer of rat cerebellar cortex (PMID: 1372043).

    • Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution + U87-MG (human glioblastoma) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 241 kDa
      Observed band size : 250 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer and concentration: 5% NFDM /TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).

    • Immunohistochemistry (Frozen sections) analysis of Rat cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilised with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counter stain. Positive staining on the molecular layer of rat cerebellar cortex (PMID: 1372043).

    • Immunohistochemistry (Frozen sections) analysis of Mouse E14 cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilised with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counter stain. Positive staining on the molecular layer of mouse E14 cerebellar cortex (PMID: 1372043).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse E14 spinal cord tissue sections labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, PH9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counter stain. Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, PH9 (ab93684). Hematoxylin was used to counter stain. A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Staining on the molecular layer of mouse cerebellar cortex (PMID: 1372043).

    • All lanes : Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

      Lane 1 : Postnatal (P0) Mouse cerebellum
      Lane 2 : Postnatal (P0) Rat brain

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 241 kDa
      Observed band size : 250 kDa (why is the actual band size different from the predicted?)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure Time:
      Lane 1: 15 seconds
      Lane 2: 30 seconds

      The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).

    • All lanes : Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

      Lane 1 : Human fetal brain
      Lane 2 : Human liver

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 241 kDa
      Observed band size : 250 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer and concentration: 5% NFDM /TBST.

      Liver is negative control (PMID: 1717349). The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).

    • Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution + Human fetal brain lysate at 10 µg

      Predicted band size : 241 kDa

    References for Anti-Tenascin C antibody [EPR4219] (ab108930)

    This product has been referenced in:
    • Yang Z  et al. Tenascin C is a prognostic determinant and potential cancer-associated fibroblasts marker for breast ductal carcinoma. Exp Mol Pathol 102:262-267 (2017). IHC-P, IF ; Human . Read more (PubMed: 28223108) »
    • Ohe R  et al. Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues. Leuk Lymphoma 57:1687-96 (2016). IHC-P ; Human . Read more (PubMed: 26700650) »

    See all 7 Publications for this product

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    All of our Western Blots are tested under reducing and denaturing conditions unless noted on the datasheet.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need a...

    Read More
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Jaculus jaculus Tissue sections (Foot section/joint)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: ProteaseK
    Permeabilization
    No
    Specification
    Foot section/joint
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Mai Tran

    Verified customer

    Submitted Jul 12 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted May 09 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (pancreatic tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH 6.0
    Permeabilization
    No
    Specification
    pancreatic tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Sep 25 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (breast tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
    Permeabilization
    No
    Specification
    breast tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Sep 25 2015

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Cow Tissue sections (Tendon)
    Permeabilization
    No
    Specification
    Tendon
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1%
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Sep 21 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (MSC)
    Permeabilization
    Yes - Triton 1%
    Specification
    MSC
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 29 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    FBS / BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample
    Human Tissue sections (Infantile fibromatosis)
    Specification
    Infantile fibromatosis
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 16 2014

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample
    Human Cell (293T)
    Specification
    293T
    Permeabilization
    Yes - Triton X-100 1%
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 04 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample
    Mouse Tissue sections (Mouse 15.5dpc)
    Specification
    Mouse 15.5dpc
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 23 2014

    1-10 of 18 Abreviews or Q&A

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