Anti-Tenascin C antibody [EPR4219] (ab108930)

Overview

  • Product nameAnti-Tenascin C antibody [EPR4219]
    See all Tenascin C primary antibodies
  • Description
    Rabbit monoclonal [EPR4219] to Tenascin C
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Tenascin C.

  • Positive control
    • Human testis tissue; Human fetal brain lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-Tenascin C antibody (HRP) [EPR4219] (ab196918)

Properties

Applications

Our Abpromise guarantee covers the use of ab108930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 241 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 24384131

Antigen retrieval is recommended.

Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC or IP.
  • Target

    • FunctionExtracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
    • Sequence similaritiesBelongs to the tenascin family.
      Contains 15 EGF-like domains.
      Contains 1 fibrinogen C-terminal domain.
      Contains 15 fibronectin type-III domains.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • 150 225 antibody
      • Cytotactin antibody
      • Glioma associated extracellular matrix antigen antibody
      • Glioma-associated-extracellular matrix antigen antibody
      • GMEM antibody
      • GP 150 225 antibody
      • GP 150-225 antibody
      • GP antibody
      • Hexabrachion antibody
      • HXB antibody
      • JI antibody
      • MGC167029 antibody
      • Miotendinous antigen antibody
      • Myotendinous antigen antibody
      • Neuronectin antibody
      • TENA_HUMAN antibody
      • Tenascin antibody
      • Tenascin-C antibody
      • Tenascin-C isoform 14/AD1/16 antibody
      • TenascinC antibody
      • TN antibody
      • TN C antibody
      • TN-C antibody
      • TNC antibody
      see all

    Anti-Tenascin C antibody [EPR4219] images

    • Overlay histogram showing SH-SY5Y cells stained with ab108930 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108930, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution + Human fetal brain lysate at 10 µg

      Predicted band size : 241 kDa

    References for Anti-Tenascin C antibody [EPR4219] (ab108930)

    This product has been referenced in:
    • Fu H  et al. Tenascin-C Is a Major Component of the Fibrogenic Niche in Kidney Fibrosis. J Am Soc Nephrol N/A:N/A (2016). Read more (PubMed: 27612995) »
    • Govindarajan P  et al. Bone matrix, cellularity, and structural changes in a rat model with high-turnover osteoporosis induced by combined ovariectomy and a multiple-deficient diet. Am J Pathol 184:765-77 (2014). IHC ; Rat . Read more (PubMed: 24384131) »

    See all 4 Publications for this product

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    Read More
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Jaculus jaculus Tissue sections (Foot section/joint)
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: ProteaseK
    Permeabilization No
    Specification Foot section/joint
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Mai Tran

    Verified customer

    Submitted Jul 12 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (skin)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization No
    Specification skin
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted May 09 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (pancreatic tumor)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH 6.0
    Permeabilization No
    Specification pancreatic tumor
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Sep 25 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (breast tumor)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
    Permeabilization No
    Specification breast tumor
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Sep 25 2015

    Application Immunohistochemistry (Frozen sections)
    Sample Cow Tissue sections (Tendon)
    Permeabilization No
    Specification Tendon
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Sep 21 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (MSC)
    Permeabilization Yes - Triton 1%
    Specification MSC
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 29 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step FBS / BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample Human Tissue sections (Infantile fibromatosis)
    Specification Infantile fibromatosis
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 16 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample Human Cell (293T)
    Specification 293T
    Permeabilization Yes - Triton X-100 1%
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 04 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample Mouse Tissue sections (Mouse 15.5dpc)
    Specification Mouse 15.5dpc
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 23 2014

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