The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: ready to use.
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
Belongs to the tenascin family. Contains 15 EGF-like domains. Contains 1 fibrinogen C-terminal domain. Contains 15 fibronectin type-III domains.
Secreted > extracellular space > extracellular matrix.