Overview

  • Product nameAnti-Tenascin C antibody [T2H5]
    See all Tenascin C primary antibodies
  • Description
    Mouse monoclonal [T2H5] to Tenascin C
  • Tested applicationsSuitable for: IHC-P, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified from a homogenate of a mammary tumour specimen) (Human).

  • Positive control
    • Human tonsil tissue
  • General notes


    Tenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from hybridoma culture supernatant by protein A-affinity chromatography.
  • Primary antibody notesTenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted.
  • ClonalityMonoclonal
  • Clone numberT2H5
  • Myelomaunknown
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 240 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 20435685

Target

  • FunctionExtracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
  • Sequence similaritiesBelongs to the tenascin family.
    Contains 15 EGF-like domains.
    Contains 1 fibrinogen C-terminal domain.
    Contains 15 fibronectin type-III domains.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 150 225 antibody
    • Cytotactin antibody
    • Glioma associated extracellular matrix antigen antibody
    • Glioma-associated-extracellular matrix antigen antibody
    • GMEM antibody
    • GP 150 225 antibody
    • GP 150-225 antibody
    • GP antibody
    • Hexabrachion antibody
    • HXB antibody
    • JI antibody
    • MGC167029 antibody
    • Miotendinous antigen antibody
    • Myotendinous antigen antibody
    • Neuronectin antibody
    • TENA_HUMAN antibody
    • Tenascin antibody
    • Tenascin-C antibody
    • Tenascin-C isoform 14/AD1/16 antibody
    • TenascinC antibody
    • TN antibody
    • TN C antibody
    • TN-C antibody
    • TNC antibody
    see all

Anti-Tenascin C antibody [T2H5] images

  • ab3970 staining paraffin embedded tissue sections.

  • Anti-Tenascin C antibody [T2H5] (ab3970) at 1 µg/ml + U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 265 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
    Tenascin C contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References for Anti-Tenascin C antibody [T2H5] (ab3970)

This product has been referenced in:
  • Estany S  et al. Lung fibrotic tenascin-C upregulation is associated with other extracellular matrix proteins and induced by TGFß1. BMC Pulm Med 14:120 (2014). WB, IHC-P ; Human . Read more (PubMed: 25064447) »
  • Fouda GG  et al. Tenascin-C is an innate broad-spectrum, HIV-1-neutralizing protein in breast milk. Proc Natl Acad Sci U S A 110:18220-5 (2013). WB . Read more (PubMed: 24145401) »

See all 9 Publications for this product

Product Wall

Te agradezco mucho que te hayas tomado el tiempo para enviarnos los detalles e imágenes del protocolo.  Lamentablemente no puedo abrir los archivos de las imágenes, por lo que te agradecería que nos las volvieras a enviar.
Me comentabas en tu prim...

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Gracias por contactarnos.

Siento mucho que estéis teniendo problemas con este anticuerpo. He comprobado nuestro registro de reclamaciones de otros clientes, así como los resultados de los controles de calidad realizados, y no pa...

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Muchas gracias por tu respuesta.

Es importante que todos los anticuerpos que escojas para llevar a cabo IHC-P en tejido humano estén validados (y por tanto garantizados por Abcam) para funcionar en esta especie y aplicación. Est...

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Thank you for your patience. I have contacted the originators of ab3970, ab6393, and ab7839 and have received the following replies: ab3970 - This antibody reacts with tenascin; it was raised against mammary tumor of human origin. Tenascin-C cou...

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Thank you for your recent phone call and your interest in our tenascin and myosin heavy chain antibodies. As we discussed we do not currently have antibodies which have been tested in chicken tissue and the homology searches revealed that for tenas...

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Thank you for the details of your protocol, my apologies for the delay due to a long bank holiday weekend in the UK. This antibody has been tested in IHC on formalin-fixed paraffin embedded tissue, I would therefore suggest using formalin fixation as i...

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Thank you for your enquiry. Unfortunatly the epitope has not been mapped yet. My apologies for the inconvenience,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"