Overview

  • Product name
  • Description
    Rabbit polyclonal to tenomodulin
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Sheep, Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (GEKKKIYMEI DPVTRTEIFR SGNGTDETLE VHDFKNGYTG IYFVGLQKCF) of Human tenomodulin (NP_071427).

  • Positive control
    • Fetal heart lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-tenomodulin antibody images

  • Anti-tenomodulin antibody (ab81328) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References for Anti-tenomodulin antibody (ab81328)

This product has been referenced in:
  • Tong WY  et al. Functional replication of the tendon tissue microenvironment by a bioimprinted substrate and the support of tenocytic differentiation of mesenchymal stem cells. Biomaterials 33:7686-98 (2012). WB ; Human . Read more (PubMed: 22818988) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (GFP+ Olfactory Stem Cells)
Permeabilization
Yes - 0,1% Triton X100
Specification
GFP+ Olfactory Stem Cells
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

Submitted Jun 10 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Olfactory Stem Cells of Rabbit)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory Stem Cells of Rabbit
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Username

Antoine Veron

Verified customer

Submitted May 27 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tendon)
Antigen retrieval step
None
Permeabilization
No
Specification
tendon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
zinc buffered formalin
Username

Abcam user community

Verified customer

Submitted Nov 03 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Olfactory stem cells (tenocytes differentiation))
Permeabilization
Yes - 0,1 % Triton X100
Specification
Olfactory stem cells (tenocytes differentiation)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

Submitted Aug 11 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Olfactory stem cells (differenciation))
Permeabilization
Yes - 0,1% Triton 100X
Specification
Olfactory stem cells (differenciation)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

Submitted Jul 16 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (E14.5 Cryosections)
Specification
E14.5 Cryosections
Permeabilization
Yes - 0.1% Tween-20
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Sheep Cell lysate - whole cell (Tendon cells derived from enzymic digestion of who)
Loading amount
10 µg
Specification
Tendon cells derived from enzymic digestion of who
Gel Running Conditions
Non-reduced Denaturing (4-20% Novex Tris-Glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. John Wardale

Verified customer

Submitted Mar 22 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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