Anti-Testin antibody (ab78499)


  • Product nameAnti-Testin antibody
    See all Testin primary antibodies
  • Description
    Rabbit polyclonal to Testin
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Testin .

    (Peptide available as ab86628.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; K562.



Our Abpromise guarantee covers the use of ab78499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).


Anti-Testin antibody images

  • All lanes : Anti-Testin antibody (ab78499) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 48 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab78499 stained HeLa cells. The cells were 10% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78499, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (10 min) HeLa, Hek293 and HepG2 cells at 5µg/ml.

References for Anti-Testin antibody (ab78499)

ab78499 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

I have heard back from the lab regarding ab102716 and they did not find any significant homology with c. elegans. Therefore, they do not recommend using this antibody with this species.

I BLASTed the immu...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 40 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 01 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Apr 08 2010